Detailed information of TA system TA211923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 837587..838196
• Replicon: chromosome
• Accession: NZ_CP080467
• Organism: Alicyclobacillus acidoterrestris strain DSM 3922

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: T0BTP7
• Locus tag: K1I37_RS03890
• Protein ID: WP_021297381.1
• Coordinates: 837846..838196 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A9E7CXD1
• Locus tag: K1I37_RS03885
• Protein ID: WP_031218972.1
• Coordinates: 837587..837841 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1I37_RS03860 (K1I37_03860)	832650..832838	-	189	WP_021297375.1	hypothetical protein	-
K1I37_RS03865 (K1I37_03865)	832969..833349	+	381	WP_021297376.1	holo-ACP synthase	-
K1I37_RS03870 (K1I37_03870)	833433..834932	+	1500	WP_021297377.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
K1I37_RS03875 (K1I37_03875)	834969..835967	+	999	WP_236025988.1	outer membrane lipoprotein carrier protein LolA	-
K1I37_RS03880 (K1I37_03880)	836231..837436	+	1206	WP_021297379.1	alanine racemase	-
K1I37_RS03885 (K1I37_03885)	837587..837841	+	255	WP_031218972.1	ribbon-helix-helix protein, CopG family	Antitoxin
K1I37_RS03890 (K1I37_03890)	837846..838196	+	351	WP_021297381.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K1I37_RS03895 (K1I37_03895)	838343..839596	+	1254	WP_021297382.1	adenosylhomocysteinase	-
K1I37_RS03900 (K1I37_03900)	839628..840365	+	738	WP_021297383.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
K1I37_RS03905 (K1I37_03905)	840537..840773	-	237	WP_152498825.1	hypothetical protein	-
K1I37_RS03910 (K1I37_03910)	840877..841206	+	330	WP_021297385.1	PadR family transcriptional regulator	-
K1I37_RS03915 (K1I37_03915)	841206..842540	+	1335	WP_021297386.1	permease prefix domain 1-containing protein	-
K1I37_RS03920 (K1I37_03920)	842728..842934	+	207	WP_021297387.1	DUF3311 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12889.03 Da
• Isoelectric Point: 6.4656

>T211923 WP_021297381.1 NZ_CP080467:837846-838196 [Alicyclobacillus acidoterrestris]
MNVKRGDIFFADLSPVVGSEQGGFRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIPAQKYGLERDSVVLLEQL
RTLDKQRLTDKITHLDDTMMKMVNDGLLISLGLVDF

Nucleotide

• Length: 351 bp

>T211923 NZ_CP106849:c3187627-3187211 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9566.83 Da
• Isoelectric Point: 4.8172

>AT211923 WP_031218972.1 NZ_CP080467:837587-837841 [Alicyclobacillus acidoterrestris]
MVVYVPEQLLEQVDGFARTDSANRSEIVREALRMYVTAHRKHEIREQMQQGYQEMAGLNLRIASESFLLEQEAGGTVERL
VSGV

Nucleotide

• Length: 255 bp

>AT211923 NZ_CP106849:c3187874-3187608 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: T0BTP7