Detailed information of TA system TA211904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 28489..29182
• Replicon: plasmid unnamed
• Accession: NZ_CP080464
• Organism: Vibrio cholerae strain ICDC-VC702

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: K1T74_RS19630
• Protein ID: WP_000182276.1
• Coordinates: 28489..28845 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: K1T74_RS19635
• Protein ID: WP_001172026.1
• Coordinates: 28847..29182 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T74_RS19600	24308..24940	+	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
K1T74_RS19605	25097..25444	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
K1T74_RS19610	25438..26277	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
K1T74_RS19615	26405..26905	+	501	WP_000376623.1	GNAT family N-acetyltransferase	-
K1T74_RS19985	27226..27294	+	69	Protein_29	hypothetical protein	-
K1T74_RS19620	27412..28176	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
K1T74_RS19625	28316..28387	-	72	Protein_31	EAL domain-containing protein	-
K1T74_RS19630	28489..28845	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1T74_RS19635	28847..29182	+	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
K1T74_RS19640	29197..29532	+	336	WP_000741275.1	hypothetical protein	-
K1T74_RS19645	29556..29882	+	327	WP_000091614.1	hypothetical protein	-
K1T74_RS19650	29879..30259	+	381	WP_001054412.1	hypothetical protein	-
K1T74_RS19660	30377..31631	+	1255	Protein_37	HD-GYP domain-containing protein	-
K1T74_RS19665	32047..32307	+	261	WP_011191337.1	hypothetical protein	-
K1T74_RS19670	32300..32695	+	396	WP_011191338.1	helix-turn-helix transcriptional regulator	-
K1T74_RS19675	32760..33329	-	570	WP_011191339.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS2 / aac(6')-Ib-cr / blaOXA-1 / catB3 / qacE / sul1	-	1..33998	33998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T211904 WP_000182276.1 NZ_CP080464:28489-28845 [Vibrio cholerae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T211904 NZ_CP106838:587364-587582 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT211904 WP_001172026.1 NZ_CP080464:28847-29182 [Vibrio cholerae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT211904 NZ_CP106838:586963-587337 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7