Detailed information of TA system TA211901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 224170..224698
• Replicon: chromosome
• Accession: NZ_CP080463
• Organism: Vibrio cholerae strain ICDC-VC702

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0X1KZS6
• Locus tag: K1T74_RS15730
• Protein ID: WP_000221354.1
• Coordinates: 224411..224698 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0X1KZS8
• Locus tag: K1T74_RS15725
• Protein ID: WP_001250179.1
• Coordinates: 224170..224421 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T74_RS15695	219714..220352	+	639	WP_220106507.1	HD domain-containing protein	-
K1T74_RS15700	220518..221258	+	741	WP_172781623.1	PhzF family phenazine biosynthesis protein	-
K1T74_RS15705	221412..221951	+	540	WP_001898634.1	histidine phosphatase family protein	-
K1T74_RS15710	222086..222622	+	537	WP_000644499.1	nucleotidyltransferase family protein	-
K1T74_RS15715	222760..223359	+	600	WP_220106436.1	glutathione S-transferase N-terminal domain-containing protein	-
K1T74_RS15720	223544..223957	+	414	WP_000049417.1	VOC family protein	-
K1T74_RS15725	224170..224421	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K1T74_RS15730	224411..224698	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1T74_RS15735	224908..225150	+	243	WP_000107462.1	hypothetical protein	-
K1T74_RS15740	225325..225714	+	390	WP_001081302.1	hypothetical protein	-
K1T74_RS15745	225850..226275	+	426	WP_002045007.1	GNAT family N-acetyltransferase	-
K1T74_RS15750	226450..227322	+	873	Protein_274	DUF3800 domain-containing protein	-
K1T74_RS15755	227472..228071	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
K1T74_RS15760	228227..228763	+	537	WP_000469484.1	GNAT family protein	-
K1T74_RS19920	228820..228891	+	72	Protein_277	iron transporter	-
K1T74_RS15765	229303..229572	+	270	WP_001114075.1	DUF4160 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	146834..233648	86814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10991.96 Da
• Isoelectric Point: 10.4934

>T211901 WP_000221354.1 NZ_CP080463:224411-224698 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Nucleotide

• Length: 288 bp

>T211901 NZ_CP106835:2179-2445 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATTAAGAATTCAGCGAAGACAGATTTAAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATAATCCATATCAACAAAGTCAATCCTTTGAAAAATTGCACCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAACTTGGTCTCATTATGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9136.29 Da
• Isoelectric Point: 4.0197

>AT211901 WP_001250179.1 NZ_CP080463:224170-224421 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Nucleotide

• Length: 252 bp

>AT211901 NZ_CP106835:1922-2179 [Staphylococcus epidermidis]
ATGAAAAGTAAAACACCAACAGAAGCTCGTAAAGATTTTTATCAGTTATTAAAACGTGTGAATAATGACCATGAACCAGT
ATATATTGAAGGCAAATATGATGAGAGTAATGCTGTTATTATAGGCATGGAAGATTGGCGTAGCATTAATGAAACGATAT
ATCTTGAGGCTACAGGCACAATGGACAAAGTTAGAGAACGTGAGCATGACAACAGTGGTTATACAAACATAGATGATATT
GATTGGGCTAGACTTTAA

Structures

Toxin

• AlphaFold DB: A0A0X1KZS6

Antitoxin

• AlphaFold DB: A0A0X1KZS8