Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5508664..5509275 Replicon chromosome
Accession NC_007005
Organism Pseudomonas syringae pv. syringae B728a

Toxin (Protein)


Gene name PfiT Uniprot ID Q4ZME8
Locus tag PSYR_RS24095 Protein ID WP_003304103.1
Coordinates 5508664..5509014 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q4ZME7
Locus tag PSYR_RS24100 Protein ID WP_002556076.1
Coordinates 5509027..5509275 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSYR_RS24075 (Psyr_4642) 5504338..5506824 + 2487 WP_011269142.1 bifunctional diguanylate cyclase/phosphodiesterase -
PSYR_RS24085 (Psyr_4643) 5507007..5508014 + 1008 Protein_4670 P-type conjugative transfer protein TrbL -
PSYR_RS24090 5508082..5508297 + 216 Protein_4671 hypothetical protein -
PSYR_RS24095 (Psyr_4644) 5508664..5509014 - 351 WP_003304103.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PSYR_RS24100 (Psyr_4645) 5509027..5509275 - 249 WP_002556076.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
PSYR_RS24105 (Psyr_4646) 5509613..5510575 + 963 WP_103714631.1 tyrosine-type recombinase/integrase -
PSYR_RS24110 (Psyr_4647) 5510601..5510915 - 315 WP_162884967.1 hypothetical protein -
PSYR_RS24115 (Psyr_4648) 5510988..5511227 - 240 WP_011269146.1 hypothetical protein -
PSYR_RS24120 (Psyr_4649) 5511307..5511753 - 447 WP_162886078.1 GNAT family N-acetyltransferase -
PSYR_RS24125 (Psyr_4650) 5511809..5512552 - 744 Protein_4678 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5511809..5512489 680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-98)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12973.76 Da        Isoelectric Point: 4.7690

>T21188 WP_003304103.1 NC_007005:c5509014-5508664 [Pseudomonas syringae pv. syringae B728a]
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSHQLSELGVLHYRELNTDGYRIFY
EVRDAGDINVIVVALVLGSKQSVEQALIRYCLLQAI

Download         Length: 351 bp

>T21188 NC_007005:c5509014-5508664 [Pseudomonas syringae pv. syringae B728a]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGTATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGTT
ACCCGGTCAGTCATCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGAGGGACGCAGGCGATATAAATGTCATCGTCGTGGCGCTCGTGTTAGGTAGCAAGCAAAGTGTTGAACAGGCCCT
CATCCGCTACTGCCTGCTTCAGGCTATATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9366.73 Da        Isoelectric Point: 9.9579

>AT21188 WP_002556076.1 NC_007005:c5509275-5509027 [Pseudomonas syringae pv. syringae B728a]
MRVETISFLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFT
QK

Download         Length: 249 bp

>AT21188 NC_007005:c5509275-5509027 [Pseudomonas syringae pv. syringae B728a]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9GDS9

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9GM89

References