Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DhiAT/dnstrm_HI1420(antitoxin)
Location 1712724..1713477 Replicon chromosome
Accession NZ_CP080400
Organism Dickeya fangzhongdai strain Onc5

Toxin (Protein)


Gene name dhiT Uniprot ID A0A2K8QMG8
Locus tag K0H75_RS07490 Protein ID WP_038918519.1
Coordinates 1712724..1712981 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID A0A2K8QKC0
Locus tag K0H75_RS07495 Protein ID WP_038918520.1
Coordinates 1712989..1713477 (+) Length 163 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H75_RS07480 (K0H75_07470) 1709499..1711649 - 2151 WP_038918518.1 formate dehydrogenase subunit alpha -
K0H75_RS07485 (K0H75_07475) 1711684..1712226 - 543 WP_013317225.1 4Fe-4S dicluster domain-containing protein -
K0H75_RS07490 (K0H75_07480) 1712724..1712981 + 258 WP_038918519.1 DUF4160 domain-containing protein Toxin
K0H75_RS07495 (K0H75_07485) 1712989..1713477 + 489 WP_038918520.1 addiction module antitoxin Antitoxin
K0H75_RS07500 (K0H75_07490) 1713562..1714059 - 498 WP_038918521.1 hydrogenase maturation peptidase HycI -
K0H75_RS07505 (K0H75_07495) 1714052..1714450 - 399 WP_038660229.1 formate hydrogenlyase maturation HycH family protein -
K0H75_RS07510 (K0H75_07500) 1714447..1715238 - 792 WP_038918522.1 NADH-quinone oxidoreductase subunit B family protein -
K0H75_RS07515 (K0H75_07505) 1715235..1715804 - 570 WP_038918524.1 hydrogenase 4 subunit H -
K0H75_RS07520 (K0H75_07510) 1715815..1717542 - 1728 WP_038918525.1 hydrogenase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Uncharacterized protein HI1420

(79-157)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9977.52 Da        Isoelectric Point: 8.1489

>T211846 WP_038918519.1 NZ_CP080400:1712724-1712981 [Dickeya fangzhongdai]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNNKRKLALAHIRAHREQLLSMWERAVVGR
NPGKL

Download         Length: 258 bp

>T211846 NZ_CP106787:c78046-77741 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 163 a.a.        Molecular weight: 17940.51 Da        Isoelectric Point: 6.9795

>AT211846 WP_038918520.1 NZ_CP080400:1712989-1713477 [Dickeya fangzhongdai]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYQYASSPVFSNASLFIKFGLLPDGSLEWSPDIKISASELRKKGEYVSHTLA
VREKNFADVISRAFYDAVIENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Download         Length: 489 bp

>AT211846 NZ_CP106787:c78266-78048 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K8QMG8

Antitoxin

Source ID Structure
AlphaFold DB A0A2K8QKC0

References