Detailed information of TA system TA211841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 575439..576220
• Replicon: chromosome
• Accession: NZ_CP080400
• Organism: Dickeya fangzhongdai strain Onc5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: K0H75_RS02660
• Protein ID: WP_038917749.1
• Coordinates: 575729..576220 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A1Y0L5F0
• Locus tag: K0H75_RS02655
• Protein ID: WP_038917748.1
• Coordinates: 575439..575732 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H75_RS02635 (K0H75_02630)	571449..572561	+	1113	WP_038917743.1	glycosyltransferase family 4 protein	-
K0H75_RS02640 (K0H75_02635)	572580..572987	+	408	WP_038917744.1	hypothetical protein	-
K0H75_RS02645 (K0H75_02640)	573026..574420	+	1395	WP_038917746.1	DUF4832 domain-containing protein	-
K0H75_RS02650 (K0H75_02645)	574447..574947	+	501	WP_038917747.1	serine acetyltransferase	-
K0H75_RS02655 (K0H75_02650)	575439..575732	+	294	WP_038917748.1	DUF1778 domain-containing protein	Antitoxin
K0H75_RS02660 (K0H75_02655)	575729..576220	+	492	WP_038917749.1	GNAT family N-acetyltransferase	Toxin
K0H75_RS02665 (K0H75_02660)	576379..576621	+	243	WP_071605145.1	hypothetical protein	-
K0H75_RS02670 (K0H75_02665)	576834..580916	+	4083	WP_038917751.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	575183..589401	14218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (75-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17628.53 Da
• Isoelectric Point: 8.2830

>T211841 WP_038917749.1 NZ_CP080400:575729-576220 [Dickeya fangzhongdai]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCNDSKVLAYYSLASSVVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SVI

Nucleotide

• Length: 492 bp

>T211841 NZ_CP106786:4070499-4070717 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATATGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10872.44 Da
• Isoelectric Point: 10.1968

>AT211841 WP_038917748.1 NZ_CP080400:575439-575732 [Dickeya fangzhongdai]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT211841 NZ_CP106786:4070098-4070472 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Y0L5F0