Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 64034..64635 Replicon plasmid pPK5086-95kb
Accession NZ_CP080373
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag K0H55_RS24195 Protein ID WP_001216045.1
Coordinates 64034..64414 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag K0H55_RS24200 Protein ID WP_001190712.1
Coordinates 64414..64635 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS24170 (K0H55_24170) 59475..60959 - 1485 WP_000124150.1 terminase -
K0H55_RS24175 (K0H55_24175) 60959..62152 - 1194 WP_029487616.1 hypothetical protein -
K0H55_RS24180 (K0H55_24180) 62238..62690 - 453 WP_001326849.1 late promoter-activating protein -
K0H55_RS24185 (K0H55_24185) 62779..63822 - 1044 WP_219903780.1 DUF968 domain-containing protein -
K0H55_RS24190 (K0H55_24190) 63850..64029 - 180 WP_000113019.1 hypothetical protein -
K0H55_RS24195 (K0H55_24195) 64034..64414 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K0H55_RS24200 (K0H55_24200) 64414..64635 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K0H55_RS24205 (K0H55_24205) 64708..65097 - 390 WP_000506730.1 S24 family peptidase -
K0H55_RS24675 65449..65856 + 408 WP_232377379.1 hypothetical protein -
K0H55_RS24225 (K0H55_24225) 67289..67651 - 363 WP_001261543.1 hypothetical protein -
K0H55_RS24230 (K0H55_24230) 67648..68580 - 933 WP_104989460.1 hypothetical protein -
K0H55_RS24235 (K0H55_24235) 68562..68936 - 375 WP_023356289.1 hypothetical protein -
K0H55_RS24240 (K0H55_24240) 68943..69236 - 294 WP_000269004.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95348 95348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T211827 WP_001216045.1 NZ_CP080373:c64414-64034 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T211827 NZ_CP106785:4420419-4420727 [Pseudomonas tohonis]
ATGATCAAGGTCCTCCAGAGCAGGGCGTTCGCCACTTGGCTGAACCACCTGAGCGACGGCAAGGCCAGGGCACGGATACT
GGCTCGCATCGACCGCATGGCCGACGGCCACCTGGGGGACGTCGCACCGATAGGCGAAGGCCTCAGCGAAGCCCGGCTCC
ACTTCGGCCCCGGCTACCGGCTCTACTTTCTCCAGCGCGGCCGGTTCCTGATCGTCCTCCTCTGCGGTGGCGACAAGGGC
AGCCAGGGGCGAGATATCGGACACGCCAGGCGCATCGCCGCAGCCTGGAAGGAGCAACACCATGACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT211827 WP_001190712.1 NZ_CP080373:c64635-64414 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT211827 NZ_CP106785:4420720-4421016 [Pseudomonas tohonis]
ATGACTGAAGACTTTTCCCGCTGGGACTCCGCCGACTACCTGCGTAGCGAGGAAGACATGGCCAACTACCTGGATGCCTG
CATCGAGGAAGCCGGCGACGATCCCGCCTTCATCGCCAAGGCCCTCGGCACCCTCGCCCGCGCCAAGGGCATGACCCAGG
TCGCCCGCGACACCGGCCTCTCGCGCGAGAGCCTGTACCGCGCGCTGTCCGGCGAGGGCAATCCCGAGTTCGGCACCATC
CTCAAGGTCCTCAGGGCACTCGGCCTGCAACTGCACGCCAGCCCCGCCCGCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References