Detailed information of TA system TA211826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 71840..72441
• Replicon: plasmid pPK5086-97kb
• Accession: NZ_CP080372
• Organism: Escherichia coli strain PK5086

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: K0H55_RS23715
• Protein ID: WP_001216034.1
• Coordinates: 72061..72441 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: K0H55_RS23710
• Protein ID: WP_001190712.1
• Coordinates: 71840..72061 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H55_RS23700 (68821)	68821..70104	-	1284	WP_001617890.1	restriction endonuclease subunit S	-
K0H55_RS23705 (70101)	70101..71657	-	1557	WP_001553856.1	type I restriction-modification system subunit M	-
K0H55_RS23710 (71840)	71840..72061	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K0H55_RS23715 (72061)	72061..72441	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K0H55_RS23720 (72446)	72446..72625	+	180	WP_001513661.1	hypothetical protein	-
K0H55_RS23725 (72653)	72653..72931	+	279	Protein_78	pdcB	-
K0H55_RS23730 (72936)	72936..73349	+	414	Protein_79	integrase core domain-containing protein	-
K0H55_RS23735 (74145)	74145..75122	+	978	WP_000361610.1	RepB family plasmid replication initiator protein	-
K0H55_RS23740 (75407)	75407..76147	-	741	WP_001066942.1	tyrosine-type recombinase/integrase	-
K0H55_RS23745 (76268)	76268..76450	-	183	WP_042065278.1	hypothetical protein	-
K0H55_RS24660 (76517)	76517..76806	-	290	Protein_83	type II toxin-antitoxin system RelE/ParE family toxin	-
K0H55_RS23750 (76793)	76793..77061	-	269	Protein_84	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA5 / aac(3)-IId	-	1..97614	97614
	-	flank	IS/Tn	-	-	72975..73349	374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T211826 WP_001216034.1 NZ_CP080372:72061-72441 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T211826 NZ_CP106785:c3932255-3931884 [Pseudomonas tohonis]
GTGCTGCTCTATCTGCTGTCCGCCGATCCGGCCAAGGCGGATGCCGCCGAAGCATTGCTGGCGAAGAGGCCGACGATCAG
CGTGCAGGTTCTCAATGAGGTGGCCAGCGTCTGCTCCCGCAAGTTGAGGATGAGCTGGGATGAGATCGGCCGTTTCCTTG
AACTGGTGCAAGGCTTCTGTCGCGTCGTACCGGTGACGCTGGAGATCCACCGGCAGGCGCGTGAGCTGGCTGAGCGCTAC
AGGCTGTCTTTCTACGATGCCTGTATCGCCGCCGCTGCGCTTGTCGCGGGCTGCTCGACCCTCCACAGCGAAGACATGCA
CTCGGGGTTGCTCCTCGATGGGCGTTTGCGGGTGCTGAATCCGTTCGGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT211826 WP_001190712.1 NZ_CP080372:71840-72061 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT211826 NZ_CP106785:c3932517-3932281 [Pseudomonas tohonis]
ATGCAAGTCGCGAAGTGGGGAAACAGCCTGGCGGTGCGTATACCGGCGAGCGTGGTCGAGGCGCTGGAACTGAAGGAGGG
TGACGATATCGAGATACTGATCGAGGGTGATCGCACCTTCGCCATCAGCAAGAAACCTGACGATGAGGGGCGGCTTGCGC
GGCTACGGGCGTTTCGCGGCAAGTTGCCGGCGGATTTCCGTTTCAACCGCGATGAGGACGCTGGCCGTGAGGACTAG

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6