Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59196..59839 Replicon plasmid pPK5086-97kb
Accession NZ_CP080372
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K0H55_RS23670 Protein ID WP_001044768.1
Coordinates 59423..59839 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K0H55_RS23665 Protein ID WP_001261287.1
Coordinates 59196..59426 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS23655 (54374) 54374..55507 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
K0H55_RS23660 (55770) 55770..58889 - 3120 WP_023909028.1 hypothetical protein -
K0H55_RS23665 (59196) 59196..59426 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K0H55_RS23670 (59423) 59423..59839 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K0H55_RS23675 (60001) 60001..62139 - 2139 WP_000350635.1 AAA family ATPase -
K0H55_RS23680 (62604) 62604..63599 + 996 WP_000246636.1 hypothetical protein -
K0H55_RS23685 (63603) 63603..64535 + 933 WP_106493967.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA5 / aac(3)-IId - 1..97614 97614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T211825 WP_001044768.1 NZ_CP080372:59423-59839 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T211825 NZ_CP106785:3650576-3650761 [Pseudomonas tohonis]
ATGAAACACAGCGAATTCAGGCGATGGTTAAAGGCCCAGGGGGTCATCTTCGAAGCGGCGAAAGGAAGCCACTTCAAGAT
CACAGCCCCCAACGGCAAGACGACGATCTTCGCGGATCACGGTGCAAAGGAGATGAAAGAACCGACCCGCAAGGCAATCA
TCAAGCAACTGGGGCTCACCGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT211825 WP_001261287.1 NZ_CP080372:59196-59426 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT211825 NZ_CP106785:3650808-3651251 [Pseudomonas tohonis]
ATGTACGACTACGCAATCCGGCTTGAGGCCGATGACACTCCAGGGTTGGCAGTCTTCTGCCGTGACCTTCCCGAGCTGAA
CAGCTATGGCGATGACGTTGCGCATGCGCTAGCTGAAGCCGTGGACGCTATCGAGTCCACCCTTTCCCTCTATGTTGACC
AGCGCCGCACGATTCCGACGGCAAGCCCCGCCGAAGCTGGCGAGCATGTGATTCGGCTTCCCGCCGTCACTGTGGCCAAG
ATCTACCTCTGGAACGCCATGATGGAACAGGGCCTGCGCAAGGCGGACCTGTGCCGCTTGCTCGGCGTCGCCCCCATGCA
GGTAGATCGCCTGGTGGACTTCCTCCACACCTCGAAGATGGAGGCTGTCGAGACCGCGCTGGCGAAGCTGGGTAAGCGGC
TGGCTGTAAGTATCGAAGCAGGGGAATGGCCGCGAAGCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References