Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52717..53242 Replicon plasmid pPK5086-97kb
Accession NZ_CP080372
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag K0H55_RS23635 Protein ID WP_001159868.1
Coordinates 52717..53022 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag K0H55_RS23640 Protein ID WP_023909027.1
Coordinates 53024..53242 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS23620 (48627) 48627..49793 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
K0H55_RS23625 (50381) 50381..51136 - 756 WP_000852146.1 replication initiation protein RepE -
K0H55_RS23630 (51910) 51910..52716 - 807 WP_000016982.1 site-specific integrase -
K0H55_RS23635 (52717) 52717..53022 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
K0H55_RS23640 (53024) 53024..53242 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K0H55_RS23645 (53876) 53876..54073 + 198 WP_000215657.1 hypothetical protein -
K0H55_RS23650 (54070) 54070..54354 - 285 WP_000642771.1 hypothetical protein -
K0H55_RS23655 (54374) 54374..55507 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA5 / aac(3)-IId - 1..97614 97614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T211824 WP_001159868.1 NZ_CP080372:c53022-52717 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T211824 NZ_CP106785:2540527-2541111 [Pseudomonas tohonis]
ATGAACATCGACACCATCAGGGCGCGGCTCGACTTCCTGCGTGAGGCGGAGCGCCTCAAGGATGTGCTGCGCAGCGCCCA
CACCTCTTCCGGGCGGGCGGAAAGCACCGCCGAGCACAGCTGGCGCCTGTGCCTGATGGCCATCACCTTCGCCGACGAGC
TGGCCGGGCTCGACCTGCTGCGGGTGCTGAAGATGTGCGTGGTGCACGACCTGGGCGAGGCCATCCATGGCGACATCCCC
GCCGTCGAGCAGGGCGGCCACCCCGACAAGGGGGCCCAGGAGCGCGAGGACCTGCTGACCCTGACCCGCACCCTGGACGA
TGGGCTGCGCGGGGAGATCCTCGCGCTGTGGGACGACTACGAGAACGCCGCCTCCCCCGAGGCGCTGGCGGTCAAGGCGC
TGGACAAGCTGGAGACCCTGCTGCAGCACACCCAGGGCGCCAACCCGGAGGACTTCGACTACGCCTTCAACCTCGGCTAC
GGGCGCCGCTACACCAGCGGCGAACCGCTGTTCGCGGCCATCCGTGCACAGCTGGACGAGGACACGAGGCGGCGGATGGA
GGCGCAGGCGAGCGCGGCTCGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT211824 WP_023909027.1 NZ_CP080372:c53242-53024 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT211824 NZ_CP106785:2539858-2540415 [Pseudomonas tohonis]
ATGCACGACAGCCTCCCGAAACCGGTCTCGCTCGGCCTCGCGCTGCGCCGTTGGCGCCTGCTGCACCGGGTGAAGCAGGC
CCACGCCGCGCAGCTGTTCGGCGTCGCCCAGTCGACCATCTCGCGCTGGGAGAGCGGGGCGCAGGCGATGGAGCCCGCCG
AGCGCGCGCGGGTGGAACAGGTGCTTGGCGCCCGGCTGGAGGCGGCGGCCGACCAGGCCCTGGCGCGGCTGGTGGGCGAC
AGCCCGCGCGCGGTGCACCTGGTCTGCGACCTGAGCCATCGCCTGCTGGCCTGTTCCGCCCTGCGCGCCGCCGAATTCAG
CGTGCCCCTCTCCGAGCTGATGGGCCGCTCGCTGTGGCGCTACGCGACGCCGGAGATCGTGCGCCAGGAAGCCGCGCTGG
ACGCCCTGGGCTGGCGCGACGTGCTGATGCCGCCGGCGCTGGAGTTCGGCAGCGGCAGCAACGAATCCGCCATCGTGCCG
ATCCGCCAGGGCCTGTGCCGCTGGACGCGCATGACGCTTTCCGACGGCACCGCCGCGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References