Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3512370..3512988 Replicon chromosome
Accession NZ_CP080370
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K0H55_RS16965 Protein ID WP_001291435.1
Coordinates 3512770..3512988 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K0H55_RS16960 Protein ID WP_000344800.1
Coordinates 3512370..3512744 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS16950 (3507459) 3507459..3508652 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K0H55_RS16955 (3508675) 3508675..3511824 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K0H55_RS16960 (3512370) 3512370..3512744 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K0H55_RS16965 (3512770) 3512770..3512988 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K0H55_RS16970 (3513160) 3513160..3513711 + 552 WP_000102568.1 maltose O-acetyltransferase -
K0H55_RS16975 (3513827) 3513827..3514297 + 471 WP_000136192.1 YlaC family protein -
K0H55_RS16980 (3514461) 3514461..3516011 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K0H55_RS16985 (3516053) 3516053..3516406 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K0H55_RS16995 (3516785) 3516785..3517096 + 312 WP_000409911.1 MGMT family protein -
K0H55_RS17000 (3517127) 3517127..3517699 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211816 WP_001291435.1 NZ_CP080370:3512770-3512988 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211816 NZ_CP106784:c1520950-1520768 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211816 WP_000344800.1 NZ_CP080370:3512370-3512744 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211816 NZ_CP106784:c1520721-1520002 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTTGCCGTTTTCTGCAGAGACTTGCCGGAACT
GAACAGCTACGGCGACGATAAGACCCATGCAATCAGCGAGGCGCTTGACGCCATCGGGACGACCCTGTCGCTCTACATTG
ACGCGCGAAAATCGATCCCGGAAGCCACTCCACCAGAGGAGGGCGAGCATGTCGTCCACCTGCCAGCCGTCACGGTGGCG
AAGATCGCGCTGTGGAACGAGATGATGAAGCGCGGAATGCGCAAGTCCGACCTGTGCAGGCTCCTGGGCATCGCCCAAAC
CCAGGGCGATCGCCTGGTGGACTTCTTGCACCACACAAAAATGGACGCCGTGGAGAGCGCCCTACTGGCACTTGGCATCC
GGCTTCGTCAATCAGTTGAGGTCAGGTTTGACGAGGTGAAACTTTACCTCGAGCGTGACACTACGGTGGTCTTATCAGAG
GCATGGTGCGAACTGAGCGCATCCTGCAAAGACGAAGCTGGAAATTATCGTGGATGGTTAAGCCTTCCAAGCCAGACATC
CCCCCAGCGGCAAACAATCAAAGGGATTGAGGCTCATCGACTCGCCTCCCACTGTCACCTCAGCATATCGGTAGATGATG
ATAGGCCTGGCGAAGCACCTTTCGAATCGCAGGGGCGAATTCACTTGCCAGTAACGTTCTACTCAGTAGAAGTAAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References