Detailed information of TA system TA211803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1344441..1345066
• Replicon: chromosome
• Accession: NZ_CP080370
• Organism: Escherichia coli strain PK5086

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K0H55_RS06545
• Protein ID: WP_000911330.1
• Coordinates: 1344668..1345066 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: K0H55_RS06540
• Protein ID: WP_000450524.1
• Coordinates: 1344441..1344668 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H55_RS06515 (1340244)	1340244..1340714	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
K0H55_RS06520 (1340714)	1340714..1341286	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
K0H55_RS06525 (1341432)	1341432..1342310	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
K0H55_RS06530 (1342327)	1342327..1343361	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
K0H55_RS06535 (1343574)	1343574..1344287	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
K0H55_RS06540 (1344441)	1344441..1344668	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K0H55_RS06545 (1344668)	1344668..1345066	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0H55_RS06550 (1345213)	1345213..1346076	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
K0H55_RS06555 (1346091)	1346091..1348106	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
K0H55_RS06560 (1348180)	1348180..1348521	+	342	Protein_1286	esterase	-
K0H55_RS06565 (1348603)	1348603..1349349	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T211803 WP_000911330.1 NZ_CP080370:1344668-1345066 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T211803 NZ_CP106775:c1654554-1654372 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT211803 WP_000450524.1 NZ_CP080370:1344441-1344668 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT211803 NZ_CP106775:c1654270-1653869 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33