Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 903465..904119 Replicon chromosome
Accession NZ_CP080370
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K0H55_RS04440 Protein ID WP_000244777.1
Coordinates 903712..904119 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K0H55_RS04435 Protein ID WP_000354046.1
Coordinates 903465..903731 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS04410 (898753) 898753..899496 + 744 Protein_870 SDR family oxidoreductase -
K0H55_RS04415 (899553) 899553..900986 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
K0H55_RS04420 (901031) 901031..901342 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
K0H55_RS04425 (901506) 901506..902165 + 660 WP_000250274.1 hemolysin III family protein -
K0H55_RS04430 (902242) 902242..903222 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K0H55_RS04435 (903465) 903465..903731 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K0H55_RS04440 (903712) 903712..904119 + 408 WP_000244777.1 protein YgfX Toxin
K0H55_RS04445 (904159) 904159..904680 - 522 WP_001055874.1 flavodoxin FldB -
K0H55_RS04450 (904792) 904792..905688 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K0H55_RS04455 (905713) 905713..906423 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K0H55_RS04460 (906429) 906429..908162 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T211800 WP_000244777.1 NZ_CP080370:903712-904119 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211800 NZ_CP106773:c1641537-1641355 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGTCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211800 WP_000354046.1 NZ_CP080370:903465-903731 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211800 NZ_CP106773:c1641253-1640852 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References