Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 318967..319553 Replicon chromosome
Accession NZ_CP080370
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag K0H55_RS01475 Protein ID WP_042014162.1
Coordinates 318967..319197 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag K0H55_RS01480 Protein ID WP_000593555.1
Coordinates 319194..319553 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS01465 (315004) 315004..317739 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
K0H55_RS01470 (317739) 317739..318863 + 1125 WP_001314210.1 ABC transporter permease -
K0H55_RS01475 (318967) 318967..319197 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
K0H55_RS01480 (319194) 319194..319553 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K0H55_RS01485 (319673) 319673..320074 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
K0H55_RS01490 (320080) 320080..320886 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
K0H55_RS01495 (320883) 320883..321647 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
K0H55_RS01500 (321647) 321647..322480 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
K0H55_RS01505 (322477) 322477..323421 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T211796 WP_042014162.1 NZ_CP080370:318967-319197 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T211796 NZ_CP106770:c334-56 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT211796 WP_000593555.1 NZ_CP080370:319194-319553 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT211796 NZ_CP106770:c519-334 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References