Detailed information of TA system TA211779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5228258..5228883
• Replicon: chromosome
• Accession: NZ_CP080362
• Organism: Klebsiella pneumoniae strain K191773

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K0P53_RS25835
• Protein ID: WP_040182550.1
• Coordinates: 5228258..5228641 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: K0P53_RS25840
• Protein ID: WP_004150355.1
• Coordinates: 5228641..5228883 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0P53_RS25820 (5225624)	5225624..5226526	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
K0P53_RS25825 (5226523)	5226523..5227158	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
K0P53_RS25830 (5227155)	5227155..5228084	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
K0P53_RS25835 (5228258)	5228258..5228641	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0P53_RS25840 (5228641)	5228641..5228883	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
K0P53_RS25845 (5229088)	5229088..5230005	+	918	WP_023302328.1	alpha/beta hydrolase	-
K0P53_RS25850 (5230019)	5230019..5230960	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
K0P53_RS25855 (5231005)	5231005..5231442	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
K0P53_RS25860 (5231439)	5231439..5232299	-	861	WP_004146232.1	virulence factor BrkB family protein	-
K0P53_RS25865 (5232293)	5232293..5232892	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T211779 WP_040182550.1 NZ_CP080362:c5228641-5228258 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T211779 NZ_CP106760:c4779257-4778724 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT211779 WP_004150355.1 NZ_CP080362:c5228883-5228641 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT211779 NZ_CP106760:c4779534-4779268 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9