Detailed information of TA system TA211762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55268..55911
• Replicon: plasmid pOXA1-191663
• Accession: NZ_CP080359
• Organism: Klebsiella pneumoniae strain K191663

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K0P45_RS27485
• Protein ID: WP_219903616.1
• Coordinates: 55268..55684 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: K0P45_RS27490
• Protein ID: WP_001261276.1
• Coordinates: 55681..55911 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0P45_RS27470 (K0P45_27455)	50312..52420	-	2109	WP_032427226.1	peptidase domain-containing ABC transporter	-
K0P45_RS27475 (K0P45_27460)	52410..53687	-	1278	WP_009309915.1	HlyD family secretion protein	-
K0P45_RS27480 (K0P45_27465)	54349..55227	+	879	WP_064182004.1	restriction endonuclease	-
K0P45_RS27485 (K0P45_27470)	55268..55684	-	417	WP_219903616.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0P45_RS27490 (K0P45_27475)	55681..55911	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K0P45_RS27495 (K0P45_27480)	56502..57446	+	945	WP_011977810.1	hypothetical protein	-
K0P45_RS27500 (K0P45_27485)	57483..57875	+	393	WP_011977811.1	hypothetical protein	-
K0P45_RS27505 (K0P45_27490)	57933..58454	+	522	WP_013214008.1	hypothetical protein	-
K0P45_RS27510 (K0P45_27495)	58500..58703	+	204	WP_011977813.1	hypothetical protein	-
K0P45_RS27515 (K0P45_27500)	58733..59737	+	1005	WP_011977814.1	hypothetical protein	-
K0P45_RS27520 (K0P45_27505)	59921..60700	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / qnrB1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IIa / tet(A)	-	1..136344	136344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15066.53 Da
• Isoelectric Point: 8.5403

>T211762 WP_219903616.1 NZ_CP080359:c55684-55268 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T211762 NZ_CP106757:c265805-265701 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT211762 WP_001261276.1 NZ_CP080359:c55911-55681 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT211762 NZ_CP106757:265513-265768 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTTAAA
AATAACCGTGCTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023