Detailed information of TA system TA211760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5283318..5283943
• Replicon: chromosome
• Accession: NZ_CP080353
• Organism: Klebsiella pneumoniae strain K191663

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K0P45_RS26060
• Protein ID: WP_040182550.1
• Coordinates: 5283318..5283701 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: K0P45_RS26065
• Protein ID: WP_004150355.1
• Coordinates: 5283701..5283943 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0P45_RS26045 (5280684)	5280684..5281586	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
K0P45_RS26050 (5281583)	5281583..5282218	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
K0P45_RS26055 (5282215)	5282215..5283144	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
K0P45_RS26060 (5283318)	5283318..5283701	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0P45_RS26065 (5283701)	5283701..5283943	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
K0P45_RS26070 (5284148)	5284148..5285065	+	918	WP_023302328.1	alpha/beta hydrolase	-
K0P45_RS26075 (5285079)	5285079..5286020	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
K0P45_RS26080 (5286065)	5286065..5286502	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
K0P45_RS26085 (5286499)	5286499..5287359	-	861	WP_004146232.1	virulence factor BrkB family protein	-
K0P45_RS26090 (5287353)	5287353..5287952	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T211760 WP_040182550.1 NZ_CP080353:c5283701-5283318 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T211760 NZ_CP106757:c261159-261016 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT211760 WP_004150355.1 NZ_CP080353:c5283943-5283701 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT211760 NZ_CP106757:260826-261084 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTTAGAAAAATAAGAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCGATTTTGTTACAA
AAAATAACCGTACTCAGTCAAAGTAAACGGTTATTTTTTCTTGTCTTCTTTAACAACCTTTAGGATTCCGAAGATTAAAG
TCGCGATAAAAGAACCGAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9