Detailed information of TA system TA211742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 9891..10642
• Replicon: plasmid pHI1B-191659
• Accession: NZ_CP080352
• Organism: Klebsiella pneumoniae strain K191659

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: K0P51_RS27025
• Protein ID: WP_014386536.1
• Coordinates: 9891..10373 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: K0P51_RS27030
• Protein ID: WP_004902250.1
• Coordinates: 10364..10642 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0P51_RS27000 (K0P51_27000)	5031..5378	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
K0P51_RS27005 (K0P51_27005)	5428..6966	+	1539	WP_134909459.1	IS66 family transposase	-
K0P51_RS27010 (K0P51_27010)	7000..7719	-	720	WP_219899820.1	DUF2971 domain-containing protein	-
K0P51_RS27015 (K0P51_27015)	7820..8212	-	393	WP_032442757.1	hypothetical protein	-
K0P51_RS27020 (K0P51_27020)	8317..8856	-	540	WP_004902239.1	hypothetical protein	-
K0P51_RS27025 (K0P51_27025)	9891..10373	-	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
K0P51_RS27030 (K0P51_27030)	10364..10642	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
K0P51_RS27035 (K0P51_27035)	10761..10973	-	213	WP_004902255.1	hypothetical protein	-
K0P51_RS27040 (K0P51_27040)	11081..11422	-	342	WP_004902257.1	hypothetical protein	-
K0P51_RS27045 (K0P51_27045)	12252..12710	-	459	WP_014386535.1	hypothetical protein	-
K0P51_RS27050 (K0P51_27050)	13363..13818	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
K0P51_RS27055 (K0P51_27055)	13890..14255	+	366	WP_004200999.1	mercuric ion transporter MerT	-
K0P51_RS27060 (K0P51_27060)	14271..14546	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
K0P51_RS27065 (K0P51_27065)	14574..14999	+	426	WP_000522996.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	armA / sul1 / qacE / aadA5 / dfrA1 / tet(B)	-	1..288525	288525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T211742 WP_014386536.1 NZ_CP080352:c10373-9891 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T211742 NZ_CP106756:c1406107-1405889 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT211742 WP_004902250.1 NZ_CP080352:c10642-10364 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT211742 NZ_CP106756:c1406507-1406133 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3