Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9677..10320 Replicon plasmid pFIA-191659
Accession NZ_CP080350
Organism Klebsiella pneumoniae strain K191659

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K0P51_RS25745 Protein ID WP_001044770.1
Coordinates 9677..10093 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K0P51_RS25750 Protein ID WP_001261282.1
Coordinates 10090..10320 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0P51_RS25720 (K0P51_25720) 5604..6524 + 921 Protein_7 IS91-like element ISCR1 family transposase -
K0P51_RS25725 (K0P51_25725) 6558..7484 - 927 Protein_8 Tn3 family transposase -
K0P51_RS25730 (K0P51_25730) 7550..8227 + 678 WP_228741390.1 IS6 family transposase -
K0P51_RS25740 (K0P51_25740) 8914..9603 - 690 Protein_11 AAA family ATPase -
K0P51_RS25745 (K0P51_25745) 9677..10093 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K0P51_RS25750 (K0P51_25750) 10090..10320 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K0P51_RS25755 (K0P51_25755) 10277..10738 + 462 WP_072202616.1 hypothetical protein -
K0P51_RS25760 (K0P51_25760) 10882..11232 + 351 WP_000493378.1 hypothetical protein -
K0P51_RS25765 (K0P51_25765) 11283..12026 + 744 WP_000129823.1 hypothetical protein -
K0P51_RS25770 (K0P51_25770) 12023..12799 + 777 WP_000015958.1 site-specific integrase -
K0P51_RS25775 (K0P51_25775) 12857..13114 - 258 WP_000764642.1 hypothetical protein -
K0P51_RS25780 (K0P51_25780) 13243..13347 - 105 WP_032409716.1 hypothetical protein -
K0P51_RS25785 (K0P51_25785) 13882..14748 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / tet(D) - 1..51884 51884


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T211740 WP_001044770.1 NZ_CP080350:c10093-9677 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T211740 NZ_CP106756:1198609-1199007 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT211740 WP_001261282.1 NZ_CP080350:c10320-10090 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT211740 NZ_CP106756:1198313-1198606 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References