Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5202495..5203120 Replicon chromosome
Accession NZ_CP080346
Organism Klebsiella pneumoniae strain K191659

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag K0P51_RS25255 Protein ID WP_019705794.1
Coordinates 5202495..5202878 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K0P51_RS25260 Protein ID WP_004150355.1
Coordinates 5202878..5203120 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0P51_RS25240 (5199861) 5199861..5200763 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K0P51_RS25245 (5200760) 5200760..5201395 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K0P51_RS25250 (5201392) 5201392..5202321 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K0P51_RS25255 (5202495) 5202495..5202878 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
K0P51_RS25260 (5202878) 5202878..5203120 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K0P51_RS25265 (5203325) 5203325..5204242 + 918 WP_004146235.1 alpha/beta hydrolase -
K0P51_RS25270 (5204257) 5204257..5205198 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K0P51_RS25275 (5205243) 5205243..5205680 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K0P51_RS25280 (5205677) 5205677..5206537 - 861 WP_004146232.1 virulence factor BrkB family protein -
K0P51_RS25285 (5206531) 5206531..5207130 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T211739 WP_019705794.1 NZ_CP080346:c5202878-5202495 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T211739 NZ_CP106756:c1192555-1192277 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT211739 WP_004150355.1 NZ_CP080346:c5203120-5202878 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT211739 NZ_CP106756:c1192818-1192558 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References