Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4586019..4586829 Replicon chromosome
Accession NZ_CP080346
Organism Klebsiella pneumoniae strain K191659

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K0P51_RS22390 Protein ID WP_004178461.1
Coordinates 4586019..4586552 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K0P51_RS22395 Protein ID WP_002887278.1
Coordinates 4586563..4586829 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0P51_RS22385 (4584850) 4584850..4585971 + 1122 WP_004186850.1 cupin domain-containing protein -
K0P51_RS22390 (4586019) 4586019..4586552 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K0P51_RS22395 (4586563) 4586563..4586829 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K0P51_RS22400 (4586932) 4586932..4588365 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
K0P51_RS22405 (4588355) 4588355..4589038 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K0P51_RS22410 (4589211) 4589211..4590596 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
K0P51_RS22415 (4590614) 4590614..4590958 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T211736 WP_004178461.1 NZ_CP080346:c4586552-4586019 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T211736 NZ_CP106756:634721-634894 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT211736 WP_002887278.1 NZ_CP080346:c4586829-4586563 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT211736 NZ_CP106756:634397-634693 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References