Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2681924..2682575 Replicon chromosome
Accession NZ_CP080336
Organism Lacticaseibacillus paracasei strain L1

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag KZR06_RS12775 Protein ID WP_003567661.1
Coordinates 2681924..2682307 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag KZR06_RS12780 Protein ID WP_003567665.1
Coordinates 2682327..2682575 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZR06_RS12770 (KZR06_12695) 2681219..2681740 - 522 WP_003567657.1 QueT transporter family protein -
KZR06_RS12775 (KZR06_12700) 2681924..2682307 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZR06_RS12780 (KZR06_12705) 2682327..2682575 - 249 WP_003567665.1 antitoxin Antitoxin
KZR06_RS12785 (KZR06_12710) 2682643..2683779 - 1137 WP_016369534.1 alanine racemase -
KZR06_RS12790 (KZR06_12715) 2683766..2684140 - 375 WP_003567669.1 holo-ACP synthase -
KZR06_RS12795 (KZR06_12720) 2684310..2685818 - 1509 WP_016387847.1 DEAD/DEAH box helicase -
KZR06_RS12800 (KZR06_12725) 2685944..2686087 - 144 WP_003588978.1 hypothetical protein -
KZR06_RS12805 (KZR06_12730) 2686118..2687506 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T211721 WP_003567661.1 NZ_CP080336:c2682307-2681924 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T211721 NZ_CP106751:c1569400-1569134 [Granulicatella adiacens]
ATGAGTTATAAACTAGTATTAAGTAATAGAGCTAGAAAGCAGTTAAAAAAAATGGATAGACTAGTATCAGTAATGTTGCT
AGATGAATTACTTAATAAGTATGATGGATTAGAAAATCCTAGACAACACGGCAAAGCCTTAAAAGGAGAATTAAAAAAAT
ATTGGCGGTATAGGATAGGAAACTATCGTATTATTTGTGATATACAAGATGACAAAATGATAGTGTTAGCCTTAGAAATA
GGACACCGTAAAGAAATATATCAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT211721 WP_003567665.1 NZ_CP080336:c2682575-2682327 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT211721 NZ_CP106751:c1569623-1569390 [Granulicatella adiacens]
ATGACGAGCAAAACATTTACAGTACGCTTAAATGAAAAAGAACAAGAGTTATTTTATGCTTATGCTAGCGTATGGGGAAA
AAGTGTAGGGGATATGATGAAAAACGCAACGCTTGAAAAAATTGAAGAATTACATGATGCACATATTGGAGATATTGCCT
ATAAAGAATTTTTGAAGAATCCACAAACATATTCATTTGATGAAGTAAGGAATGAACTTTTGAATGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References