Detailed information of TA system TA211719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 516392..517020
• Replicon: chromosome
• Accession: NZ_CP080336
• Organism: Lacticaseibacillus paracasei strain L1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S2NVS0
• Locus tag: KZR06_RS02365
• Protein ID: WP_016373562.1
• Coordinates: 516652..517020 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: KZR06_RS02360
• Protein ID: WP_003583264.1
• Coordinates: 516392..516652 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZR06_RS02330 (KZR06_02300)	512182..512712	+	531	WP_012490988.1	DNA-directed RNA polymerase subunit sigma-24	-
KZR06_RS02335 (KZR06_02305)	512880..513032	+	153	WP_003563363.1	YvrJ family protein	-
KZR06_RS02340 (KZR06_02310)	513042..513806	+	765	WP_016387929.1	GH25 family lysozyme	-
KZR06_RS02345 (KZR06_02315)	514017..514520	+	504	WP_003563372.1	TetR/AcrR family transcriptional regulator	-
KZR06_RS02350 (KZR06_02320)	514525..515586	+	1062	WP_016387927.1	ABC transporter permease	-
KZR06_RS02355 (KZR06_02325)	515591..516280	+	690	WP_016387926.1	ABC transporter ATP-binding protein	-
KZR06_RS02360 (KZR06_02330)	516392..516652	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
KZR06_RS02365 (KZR06_02335)	516652..517020	-	369	WP_016373562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZR06_RS02370 (KZR06_02340)	517312..518682	-	1371	WP_016387925.1	FAD-dependent oxidoreductase	-
KZR06_RS02375 (KZR06_02345)	519046..519864	-	819	WP_019914724.1	Cof-type HAD-IIB family hydrolase	-
KZR06_RS02380 (KZR06_02350)	520181..520450	-	270	WP_003563382.1	type II toxin-antitoxin system YafQ family toxin	-
KZR06_RS02385 (KZR06_02355)	520691..521698	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14923.01 Da
• Isoelectric Point: 8.3986

>T211719 WP_016373562.1 NZ_CP080336:c517020-516652 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMPERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQANLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T211719 NZ_CP106749:c1636400-1636218 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT211719 WP_003583264.1 NZ_CP080336:c516652-516392 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT211719 NZ_CP106749:c1636116-1635715 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Toxin

• AlphaFold DB: S2NVS0