Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3406792..3407425 Replicon chromosome
Accession NZ_CP080329
Organism Weizmannia coagulans strain VHProbi C08

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag KZO26_RS16190 Protein ID WP_013860680.1
Coordinates 3406792..3407142 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag KZO26_RS16195 Protein ID WP_014096808.1
Coordinates 3407147..3407425 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO26_RS16155 (KZO26_16155) 3402049..3402828 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
KZO26_RS16160 (KZO26_16160) 3402806..3403276 - 471 WP_014096814.1 anti-sigma B factor RsbW -
KZO26_RS16165 (KZO26_16165) 3403280..3403615 - 336 WP_026104587.1 anti-sigma factor antagonist -
KZO26_RS16170 (KZO26_16170) 3403685..3404695 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
KZO26_RS16175 (KZO26_16175) 3404709..3405110 - 402 WP_014096811.1 anti-sigma regulatory factor -
KZO26_RS16180 (KZO26_16180) 3405115..3405471 - 357 WP_014096810.1 STAS domain-containing protein -
KZO26_RS16185 (KZO26_16185) 3405475..3406302 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
KZO26_RS16190 (KZO26_16190) 3406792..3407142 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZO26_RS16195 (KZO26_16195) 3407147..3407425 - 279 WP_014096808.1 hypothetical protein Antitoxin
KZO26_RS16200 (KZO26_16200) 3407578..3408729 - 1152 WP_014096807.1 alanine racemase -
KZO26_RS16205 (KZO26_16205) 3408931..3409947 - 1017 WP_035183268.1 outer membrane lipoprotein carrier protein LolA -
KZO26_RS16210 (KZO26_16210) 3410088..3410438 - 351 WP_014096805.1 holo-ACP synthase -
KZO26_RS16215 (KZO26_16215) 3410719..3411318 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T211706 WP_013860680.1 NZ_CP080329:c3407142-3406792 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T211706 NZ_CP106744:c4830690-4829935 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT211706 WP_014096808.1 NZ_CP080329:c3407425-3407147 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT211706 NZ_CP106744:c4831193-4830825 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCTTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References