Detailed information of TA system TA211701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 13863..14328
• Replicon: plasmid unnamed1
• Accession: NZ_CP080313
• Organism: Klebsiella pneumoniae strain KP-426

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: KZ481_RS29425
• Protein ID: WP_001230707.1
• Coordinates: 13863..14114 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: KZ481_RS29430
• Protein ID: WP_000801440.1
• Coordinates: 14101..14328 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ481_RS29400 (KZ481_29405)	9301..9828	-	528	WP_000577043.1	DUF2726 domain-containing protein	-
KZ481_RS29405 (KZ481_29410)	10006..10410	-	405	WP_012421203.1	hypothetical protein	-
KZ481_RS29410 (KZ481_29415)	10423..11289	-	867	WP_001353743.1	replication initiation protein	-
KZ481_RS29415 (KZ481_29420)	12704..13219	+	516	WP_001025395.1	J domain-containing protein	-
KZ481_RS29420 (KZ481_29425)	13324..13866	+	543	WP_001083906.1	hypothetical protein	-
KZ481_RS29425 (KZ481_29430)	13863..14114	+	252	WP_001230707.1	hypothetical protein	Toxin
KZ481_RS29430 (KZ481_29435)	14101..14328	+	228	WP_000801440.1	hypothetical protein	Antitoxin
KZ481_RS29435 (KZ481_29440)	14782..15081	+	300	WP_000650309.1	hypothetical protein	-
KZ481_RS29440 (KZ481_29445)	15172..15522	+	351	WP_000854259.1	hypothetical protein	-
KZ481_RS29445 (KZ481_29450)	15827..16075	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
KZ481_RS29450 (KZ481_29455)	16072..16419	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
KZ481_RS29455 (KZ481_29460)	16780..17283	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
KZ481_RS29460 (KZ481_29465)	17287..18516	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
KZ481_RS29465 (KZ481_29470)	18593..18886	+	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
KZ481_RS29470 (KZ481_29475)	18883..19098	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31808	31808

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T211701 WP_001230707.1 NZ_CP080313:13863-14114 [Klebsiella pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T211701 NZ_CP106744:c148994-148713 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT211701 WP_000801440.1 NZ_CP080313:14101-14328 [Klebsiella pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT211701 NZ_CP106744:c149218-148991 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9