Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19892..20535 Replicon plasmid p426-Vir
Accession NZ_CP080310
Organism Klebsiella pneumoniae strain KP-426

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KZ481_RS27125 Protein ID WP_001044770.1
Coordinates 20119..20535 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KZ481_RS27120 Protein ID WP_001261282.1
Coordinates 19892..20122 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ481_RS27095 (KZ481_27100) 15832..15999 - 168 Protein_18 IS1 family transposase -
KZ481_RS27100 (KZ481_27105) 16303..17232 + 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
KZ481_RS27105 (KZ481_27110) 17377..18156 - 780 WP_004213560.1 site-specific integrase -
KZ481_RS27110 (KZ481_27115) 18153..18974 - 822 WP_004213562.1 hypothetical protein -
KZ481_RS27115 (KZ481_27120) 19519..19935 - 417 WP_164481821.1 hypothetical protein -
KZ481_RS27120 (KZ481_27125) 19892..20122 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZ481_RS27125 (KZ481_27130) 20119..20535 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ481_RS27130 (KZ481_27135) 20609..22171 + 1563 WP_004206609.1 AAA family ATPase -
KZ481_RS27135 (KZ481_27140) 22156..23178 + 1023 WP_000361404.1 helicase UvrD -
KZ481_RS27140 (KZ481_27145) 23434..24131 + 698 WP_223174630.1 IS1 family transposase -
KZ481_RS27145 (KZ481_27150) 24442..24936 + 495 WP_004213594.1 hypothetical protein -
KZ481_RS27150 (KZ481_27155) 24997..25110 + 114 Protein_29 Hha/YmoA family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..188058 188058
- inside IScluster/Tn - - 23628..38386 14758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T211692 WP_001044770.1 NZ_CP080310:20119-20535 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T211692 NZ_CP106742:c4965264-4964971 [Pseudomonas aeruginosa]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCTTTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT211692 WP_001261282.1 NZ_CP080310:19892-20122 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT211692 NZ_CP106742:c4965524-4965252 [Pseudomonas aeruginosa]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTTGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References