Detailed information of TA system TA021169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 14927..15495
• Replicon: plasmid 1
• Accession: NC_006823
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB_RS20310
• Protein ID: WP_041647774.1
• Coordinates: 14927..15298 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EB_RS20315
• Protein ID: WP_020686163.1
• Coordinates: 15295..15495 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS20285	10137..10538	-	402	WP_083783042.1	cell wall hydrolase	-
EB_RS20290	10674..11744	+	1071	WP_049780390.1	ankyrin repeat domain-containing protein	-
EB_RS20295	11807..12421	-	615	WP_011254908.1	hypothetical protein	-
EB_RS20300	12626..13606	-	981	WP_011254907.1	hypothetical protein	-
EB_RS23520	13908..14048	+	141	Protein_15	transposase	-
EB_RS24455	14243..14914	-	672	WP_041647773.1	hypothetical protein	-
EB_RS20310	14927..15298	-	372	WP_041647774.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB_RS20315	15295..15495	-	201	WP_020686163.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EB_RS20320	15772..15975	-	204	WP_011254903.1	DUF2283 domain-containing protein	-
EB_RS20325	15972..16241	-	270	WP_157866759.1	DUF4258 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207355	207355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-108)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13566.64 Da
• Isoelectric Point: 6.7686

>T21169 WP_041647774.1 NC_006823:c15298-14927 [Aromatoleum aromaticum EbN1]
MILVDTSVWIDHLRSTEAHLSELLNEGRVCMHPHVLGEIALGSLKHRAVVLEAMQNLPPAIVATDDEVFRFIDHHALFGL
GVGYTDAHLLAATKLSPGTRLWTRDRRLGSATARLGIAYELAH

Nucleotide

• Length: 372 bp

>T21169 NC_006823:c15298-14927 [Aromatoleum aromaticum EbN1]
ATGATCCTGGTGGACACCTCGGTCTGGATCGACCACTTGCGTAGCACCGAAGCGCACTTGTCCGAACTCTTGAACGAAGG
ACGGGTGTGCATGCATCCCCATGTGCTGGGCGAGATCGCACTCGGCAGTCTCAAGCATCGGGCAGTCGTGCTCGAGGCCA
TGCAGAATCTGCCGCCTGCGATCGTGGCCACCGACGACGAAGTCTTTCGCTTCATCGACCATCACGCCCTCTTCGGGCTG
GGCGTCGGCTATACCGATGCGCACCTGCTGGCAGCTACCAAGCTTTCACCGGGTACCCGGCTCTGGACGCGCGATCGGCG
CCTCGGCAGCGCAACTGCCCGGCTCGGGATCGCTTACGAATTGGCCCACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7352.46 Da
• Isoelectric Point: 7.0136

>AT21169 WP_020686163.1 NC_006823:c15495-15295 [Aromatoleum aromaticum EbN1]
MRTTLALDDELLAKAQALTGMTEKSALVKEALRALIQRESARRLALLGGSEPQLTDIPRRRPEHDE

Nucleotide

• Length: 201 bp

>AT21169 NC_006823:c15495-15295 [Aromatoleum aromaticum EbN1]
ATGCGCACCACGCTCGCGCTCGACGATGAACTTTTAGCCAAGGCCCAGGCGCTGACCGGCATGACCGAAAAATCTGCCTT
GGTGAAGGAGGCTTTGCGAGCGCTCATCCAACGCGAAAGCGCCCGCCGGCTGGCCCTGCTAGGCGGCTCAGAACCACAGC
TGACCGACATCCCGCGGCGCCGGCCGGAACACGACGAATGA