Detailed information of TA system TA211673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2104..2578
• Replicon: plasmid unnamed2
• Accession: NZ_CP080308
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K0E63_RS29610
• Protein ID: WP_004197762.1
• Coordinates: 2104..2370 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K0E63_RS29615
• Protein ID: WP_000879771.1
• Coordinates: 2354..2578 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS29595 (K0E63_29595)	721..1047	+	327	WP_050516389.1	hypothetical protein	-
K0E63_RS29600 (K0E63_29600)	1073..1618	+	546	WP_023317533.1	hypothetical protein	-
K0E63_RS29605 (K0E63_29605)	1684..2034	+	351	WP_004197756.1	hypothetical protein	-
K0E63_RS29610 (K0E63_29610)	2104..2370	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E63_RS29615 (K0E63_29615)	2354..2578	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K0E63_RS29620 (K0E63_29620)	3245..3457	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K0E63_RS29625 (K0E63_29625)	3464..3973	-	510	WP_032433972.1	MbeB family mobilization protein	-
K0E63_RS30025	4008..4442	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
K0E63_RS29635 (K0E63_29635)	4651..4995	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
K0E63_RS29640 (K0E63_29640)	4985..5161	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T211673 WP_004197762.1 NZ_CP080308:c2370-2104 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T211673 NZ_CP106682:782937-783215 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT211673 WP_000879771.1 NZ_CP080308:c2578-2354 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT211673 NZ_CP106682:783227-783532 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: S1EJF5