Detailed information of TA system TA211672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 18212..18677
• Replicon: plasmid unnamed1
• Accession: NZ_CP080307
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: K0E63_RS29515
• Protein ID: WP_001230707.1
• Coordinates: 18426..18677 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: K0E63_RS29510
• Protein ID: WP_000801440.1
• Coordinates: 18212..18439 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS29470 (K0E63_29470)	13442..13657	-	216	WP_000760375.1	EexN family lipoprotein	-
K0E63_RS29475 (K0E63_29475)	13654..13947	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
K0E63_RS29480 (K0E63_29480)	14024..15253	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
K0E63_RS29485 (K0E63_29485)	15257..15760	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
K0E63_RS29490 (K0E63_29490)	16121..16468	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
K0E63_RS29495 (K0E63_29495)	16465..16713	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
K0E63_RS29500 (K0E63_29500)	17018..17368	-	351	WP_000854259.1	hypothetical protein	-
K0E63_RS29505 (K0E63_29505)	17459..17758	-	300	WP_000650309.1	hypothetical protein	-
K0E63_RS29510 (K0E63_29510)	18212..18439	-	228	WP_000801440.1	hypothetical protein	Antitoxin
K0E63_RS29515 (K0E63_29515)	18426..18677	-	252	WP_001230707.1	hypothetical protein	Toxin
K0E63_RS29520 (K0E63_29520)	18674..19216	-	543	WP_001083906.1	hypothetical protein	-
K0E63_RS29525 (K0E63_29525)	19321..19836	-	516	WP_001025395.1	J domain-containing protein	-
K0E63_RS29530 (K0E63_29530)	21251..22117	+	867	WP_001353743.1	replication initiation protein	-
K0E63_RS29535 (K0E63_29535)	22130..22534	+	405	WP_012421203.1	hypothetical protein	-
K0E63_RS29540 (K0E63_29540)	22712..23239	+	528	WP_000577043.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31808	31808

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T211672 WP_001230707.1 NZ_CP080307:c18677-18426 [Klebsiella pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T211672 NZ_CP106682:c141461-141180 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGTAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGAAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT211672 WP_000801440.1 NZ_CP080307:c18439-18212 [Klebsiella pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT211672 NZ_CP106682:c141685-141458 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCGGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9