Detailed information of TA system TA211671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 16121..16713
• Replicon: plasmid unnamed1
• Accession: NZ_CP080307
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: K0E63_RS29495
• Protein ID: WP_000520549.1
• Coordinates: 16465..16713 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: K0E63_RS29490
• Protein ID: WP_000681613.1
• Coordinates: 16121..16468 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS29460 (K0E63_29460)	12124..12450	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
K0E63_RS29465 (K0E63_29465)	12464..13084	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
K0E63_RS29470 (K0E63_29470)	13442..13657	-	216	WP_000760375.1	EexN family lipoprotein	-
K0E63_RS29475 (K0E63_29475)	13654..13947	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
K0E63_RS29480 (K0E63_29480)	14024..15253	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
K0E63_RS29485 (K0E63_29485)	15257..15760	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
K0E63_RS29490 (K0E63_29490)	16121..16468	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K0E63_RS29495 (K0E63_29495)	16465..16713	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
K0E63_RS29500 (K0E63_29500)	17018..17368	-	351	WP_000854259.1	hypothetical protein	-
K0E63_RS29505 (K0E63_29505)	17459..17758	-	300	WP_000650309.1	hypothetical protein	-
K0E63_RS29510 (K0E63_29510)	18212..18439	-	228	WP_000801440.1	hypothetical protein	-
K0E63_RS29515 (K0E63_29515)	18426..18677	-	252	WP_001230707.1	hypothetical protein	-
K0E63_RS29520 (K0E63_29520)	18674..19216	-	543	WP_001083906.1	hypothetical protein	-
K0E63_RS29525 (K0E63_29525)	19321..19836	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31808	31808

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T211671 WP_000520549.1 NZ_CP080307:c16713-16465 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T211671 NZ_CP106681:6653648-6653926 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT211671 WP_000681613.1 NZ_CP080307:c16468-16121 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT211671 NZ_CP106681:6653938-6654243 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1