Detailed information of TA system TA211670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 48657..49237
• Replicon: plasmid pCT77-tetA
• Accession: NZ_CP080306
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K0E63_RS29195
• Protein ID: WP_071177730.1
• Coordinates: 48923..49237 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K0E63_RS29190
• Protein ID: WP_000093040.1
• Coordinates: 48657..48935 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS29160 (K0E63_29160)	43834..44595	-	762	Protein_57	Y-family DNA polymerase	-
K0E63_RS29165 (K0E63_29165)	44595..45020	-	426	WP_045325066.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
K0E63_RS29170 (K0E63_29170)	45098..46585	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
K0E63_RS29175 (K0E63_29175)	47230..47475	+	246	WP_032440458.1	hypothetical protein	-
K0E63_RS29180 (K0E63_29180)	47749..48120	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
K0E63_RS29945	48117..48482	+	366	WP_072354022.1	TonB family protein	-
K0E63_RS29190 (K0E63_29190)	48657..48935	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K0E63_RS29195 (K0E63_29195)	48923..49237	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E63_RS29200 (K0E63_29200)	49401..49829	+	429	WP_001140599.1	hypothetical protein	-
K0E63_RS29205 (K0E63_29205)	49855..50034	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K0E63_RS29210 (K0E63_29210)	50061..50591	-	531	WP_071177729.1	hypothetical protein	-
K0E63_RS29215 (K0E63_29215)	50598..51329	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
K0E63_RS29220 (K0E63_29220)	51329..53293	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / catA2 / sul2 / dfrA14	-	1..92645	92645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T211670 WP_071177730.1 NZ_CP080306:48923-49237 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T211670 NZ_CP106681:5438551-5438832 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT211670 WP_000093040.1 NZ_CP080306:48657-48935 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT211670 NZ_CP106681:5438327-5438554 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1