Detailed information of TA system TA211664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 113195..113865
• Replicon: plasmid pCT77-Vir
• Accession: NZ_CP080304
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K0E63_RS27670
• Protein ID: WP_004213072.1
• Coordinates: 113195..113638 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K0E63_RS27675
• Protein ID: WP_004213073.1
• Coordinates: 113635..113865 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS27635 (K0E63_27635)	108606..108881	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
K0E63_RS27640 (K0E63_27640)	108944..109435	+	492	WP_011251327.1	DM13 domain-containing protein	-
K0E63_RS27645 (K0E63_27645)	109484..110404	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
K0E63_RS27650 (K0E63_27650)	110495..110898	+	404	Protein_131	GAF domain-containing protein	-
K0E63_RS27655 (K0E63_27655)	111416..112051	-	636	Protein_132	mucoid phenotype regulator RmpA2	-
K0E63_RS27660 (K0E63_27660)	112468..112772	+	305	Protein_133	transposase	-
K0E63_RS29885	112795..113046	-	252	WP_186987481.1	hypothetical protein	-
K0E63_RS27670 (K0E63_27670)	113195..113638	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0E63_RS27675 (K0E63_27675)	113635..113865	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K0E63_RS27680 (K0E63_27680)	114473..115606	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K0E63_RS27685 (K0E63_27685)	115622..115915	+	294	WP_004213076.1	hypothetical protein	-
K0E63_RS27690 (K0E63_27690)	115905..116111	-	207	WP_004213077.1	hypothetical protein	-
K0E63_RS27695 (K0E63_27695)	116463..116753	+	291	WP_004213078.1	hypothetical protein	-
K0E63_RS27700 (K0E63_27700)	116743..117642	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..186446	186446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T211664 WP_004213072.1 NZ_CP080304:c113638-113195 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T211664 NZ_CP106680:c5305075-5304797 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT211664 WP_004213073.1 NZ_CP080304:c113865-113635 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT211664 NZ_CP106680:c5304785-5304480 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620