Detailed information of TA system TA021166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 202341..203011
• Replicon: plasmid pK2044
• Accession: NC_006625
• Organism: Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KP1_RS26825
• Protein ID: WP_004213072.1
• Coordinates: 202568..203011 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KP1_RS26820
• Protein ID: WP_004213073.1
• Coordinates: 202341..202571 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP1_RS26795	198564..199463	-	900	WP_004225022.1	nucleotide-binding protein	-
KP1_RS26800	199453..199743	-	291	WP_004213078.1	hypothetical protein	-
KP1_RS26805	200095..200301	+	207	WP_004213077.1	hypothetical protein	-
KP1_RS26810	200291..200584	-	294	WP_004213076.1	hypothetical protein	-
KP1_RS26815	200600..201733	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KP1_RS26820	202341..202571	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KP1_RS26825	202568..203011	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KP1_RS26830	203025..203441	-	417	Protein_214	restriction endonuclease	-
KP1_RS26835	203445..203738	-	294	Protein_215	transposase	-
KP1_RS30300	204155..204790	+	636	Protein_216	response regulator	-
KP1_RS28580	205305..205711	-	407	Protein_217	GAF domain-containing protein	-
KP1_RS26845	205802..206722	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
KP1_RS26850	206771..207262	-	492	WP_011251327.1	DM13 domain-containing protein	-
KP1_RS26855	207325..207600	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..224152	224152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T21166 WP_004213072.1 NC_006625:202568-203011 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T21166 NC_006625:202568-203011 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT21166 WP_004213073.1 NC_006625:202341-202571 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT21166 NC_006625:202341-202571 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620