Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4808452..4809262 Replicon chromosome
Accession NZ_CP080303
Organism Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K0E63_RS23855 Protein ID WP_002887280.1
Coordinates 4808452..4808985 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K0E63_RS23860 Protein ID WP_002887278.1
Coordinates 4808996..4809262 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E63_RS23850 (K0E63_23850) 4807283..4808404 + 1122 WP_002887282.1 cupin domain-containing protein -
K0E63_RS23855 (K0E63_23855) 4808452..4808985 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K0E63_RS23860 (K0E63_23860) 4808996..4809262 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K0E63_RS23865 (K0E63_23865) 4809365..4810798 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
K0E63_RS23870 (K0E63_23870) 4810788..4811471 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K0E63_RS23875 (K0E63_23875) 4811643..4813028 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
K0E63_RS23880 (K0E63_23880) 4813046..4813390 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T211658 WP_002887280.1 NZ_CP080303:c4808985-4808452 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T211658 NZ_CP106679:3353145-3353405 [Reichenbachiella sp. BKB1-1]
ATGACAAAGCTCATTAAATTTTCTTCTCATGCTTGGCTGGACTACATCCACTGGCAGAAGTCCGACAAGGAAATGAAGCA
ACTCATAGACAAGCTGTGCTTGTCTATTCTTGAAACTCCCTACCAAGGGTTGGGCTACCCCCAGCCGTTGAGCTATGAAT
TGAATCAGATCTGGTGTCGCCGTATCAACATGGAACACCGCCTGGTCTACCGCATCAATAAAGATCAGATAGAGATATTG
CAGTGTCGATTGCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT211658 WP_002887278.1 NZ_CP080303:c4809262-4808996 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT211658 NZ_CP106679:3352894-3353148 [Reichenbachiella sp. BKB1-1]
ATGGATCATACTACCTATACCAACTTCAGACAAAATTTGAAATCATTCATGGATCGGGTCATGAATGACAGGATTCCTCT
CTTGGTGACTCGCGCCCATGGACAGGATGTAGTGGTTTTATCAAAGGCAGACTTTGACAGTATTCATGAAACCTTCTACT
TATTGAAAAACCCACGAAACGCACAAAGAATATTTGAATCTTTGAGTGAACTACGAGATGGGGGAGGTTTTGAGAAATCA
ATACAAAAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References