Detailed information of TA system TA211642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1363..1943
• Replicon: plasmid pC76-coIRNAI
• Accession: NZ_CP080301
• Organism: Klebsiella pneumoniae strain KP-C76

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K0E64_RS29370
• Protein ID: WP_071177730.1
• Coordinates: 1363..1677 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K0E64_RS29375
• Protein ID: WP_000093040.1
• Coordinates: 1665..1943 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E64_RS29355 (K0E64_29355)	9..539	+	531	WP_071177729.1	hypothetical protein	-
K0E64_RS29360 (K0E64_29360)	566..745	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K0E64_RS29365 (K0E64_29365)	771..1199	-	429	WP_001140599.1	hypothetical protein	-
K0E64_RS29370 (K0E64_29370)	1363..1677	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E64_RS29375 (K0E64_29375)	1665..1943	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K0E64_RS29750	2118..2483	-	366	WP_072354022.1	TonB family protein	-
K0E64_RS29385 (K0E64_29385)	2480..2851	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
K0E64_RS29390 (K0E64_29390)	3125..3370	-	246	WP_032440458.1	hypothetical protein	-
K0E64_RS29395 (K0E64_29395)	4015..5502	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T211642 WP_071177730.1 NZ_CP080301:c1677-1363 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T211642 NZ_CP106660:c3601884-3601708 [Pantoea dispersa]
TTGAGCAGCAGGGAGCTGATTAGGATGTTGCTTGAACGAGGGTGGATTCTGGATCGTGTTAAAGGAAGCCATCATGTGTT
TGTCCGTGCAGATAAGCCATATCACATCTCGCTTCCTCATCCTGAAAAAGACCTGGCAACCGGAACATTACGTAAGCTAT
TGAAAATGATGGACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT211642 WP_000093040.1 NZ_CP080301:c1943-1665 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT211642 NZ_CP106660:c3601589-3601185 [Pantoea dispersa]
ATGAGATTCCCCGTTTATTTGCACAAAACAGAAAACGGCAGCTGGTCAGGTTTCGTACCCGACGTGCAGGGATGTTTTTT
TGCGGGCAACACCGTTGACGAGGCGTTGAGCGATGCATTTGGTGCAATCGATGCGCATGTGGAATCGTTAGCGGACGCAG
GCAAGGCCATTCCAAAAGCAGCCAGCGTTGAAACCCATATTAATGATGAAGAGTGCCAGGGAGGTTACTGGGCTTTCGTT
GAGATTGATCTCAGTCGTTACGAAGGTAAAGCGGTGAAGCTGAACATCACCCTGCCGCAAAACTTACTGACCAAAATCGA
TAGCCACGTAGAAGCACACCGCGAATACGGCTCCCGTAGCGGCTTTCTCGCCGCGCTGGCACGCAGAGAACTGGCAAACA
TCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1