Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 30617..31527 Replicon plasmid pK2044
Accession NC_006625
Organism Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KP1_RS25955 Protein ID WP_004026354.1
Coordinates 31057..31527 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag KP1_RS25950 Protein ID WP_004026357.1
Coordinates 30617..31060 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP1_RS25925 25635..26156 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
KP1_RS29070 26153..27106 + 954 Protein_28 fec operon regulator FecR -
KP1_RS25935 27193..29319 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
KP1_RS25940 29394..29549 + 156 WP_004213642.1 hypothetical protein -
KP1_RS28375 29694..29915 + 222 WP_004213643.1 DUF2188 domain-containing protein -
KP1_RS25945 30013..30516 + 504 Protein_32 DUF4113 domain-containing protein -
KP1_RS25950 30617..31060 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
KP1_RS25955 31057..31527 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KP1_RS25965 31676..32373 + 698 Protein_35 IS1 family transposase -
KP1_RS25970 33759..34708 - 950 Protein_36 DUF1173 family protein -
KP1_RS25975 34886..35380 + 495 WP_004212794.1 thermonuclease family protein -
KP1_RS25980 35717..36115 + 399 WP_004212796.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..224152 224152
- flank IS/Tn - - 31870..32373 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T21164 WP_004026354.1 NC_006625:31057-31527 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T21164 NC_006625:31057-31527 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT21164 WP_004026357.1 NC_006625:30617-31060 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT21164 NC_006625:30617-31060 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References