Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 84093..85003 Replicon plasmid pC76-Vir
Accession NZ_CP080298
Organism Klebsiella pneumoniae strain KP-C76

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag K0E64_RS27460 Protein ID WP_004026354.1
Coordinates 84093..84563 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag K0E64_RS27465 Protein ID WP_004026357.1
Coordinates 84560..85003 (-) Length 148 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E64_RS27420 (K0E64_27420) 79409..79723 + 315 WP_011251273.1 hypothetical protein -
K0E64_RS27425 (K0E64_27425) 79732..80226 + 495 WP_011251274.1 hypothetical protein -
K0E64_RS27430 (K0E64_27430) 80232..80672 + 441 WP_011251275.1 hypothetical protein -
K0E64_RS27435 (K0E64_27435) 81097..82053 - 957 WP_011251280.1 DsbA family protein -
K0E64_RS27440 (K0E64_27440) 82113..82454 - 342 WP_011251281.1 hypothetical protein -
K0E64_RS27445 (K0E64_27445) 82468..82779 - 312 WP_011251282.1 hypothetical protein -
K0E64_RS27450 (K0E64_27450) 82796..83245 - 450 WP_011251283.1 thioredoxin fold domain-containing protein -
K0E64_RS27460 (K0E64_27460) 84093..84563 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
K0E64_RS27465 (K0E64_27465) 84560..85003 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
K0E64_RS27470 (K0E64_27470) 85104..85607 - 504 Protein_97 DUF4113 domain-containing protein -
K0E64_RS27475 (K0E64_27475) 85813..86793 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
K0E64_RS27480 (K0E64_27480) 86859..88166 + 1308 Protein_99 FepA family TonB-dependent siderophore receptor -
K0E64_RS27485 (K0E64_27485) 88228..88449 + 222 WP_004213615.1 hypothetical protein -
K0E64_RS27490 (K0E64_27490) 88592..89494 - 903 WP_004213613.1 DMT family inner membrane transporter PEG344 -
K0E64_RS27495 (K0E64_27495) 89567..89968 - 402 WP_214588034.1 LuxR C-terminal-related transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..197407 197407
- inside IScluster/Tn - iroN / rmpA 77812..97788 19976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-148)

Antitoxin

(30-89)


Sequences


Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T211635 WP_004026354.1 NZ_CP080298:c84563-84093 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T211635 NZ_CP106659:c66605-66120 [Klebsiella michiganensis]
GTGGGACATGTAACAGCACCAGAACCTCTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAAACTGTGCTCGA
TGACTGGTTAAAGCAGAAAGGCCTTAAAAACCAGTCTCTCGGAGCGGCCAGAACGTTTGTGGTATGTAAGAAAGATACGA
AGCAAATAGCCGGCTTTTACTCTCTGGCCACCGGGAGCGTCAATCATACTGAAGCGACAGGGAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGCCTTGCTGTCGATCTTTCATTGCGCGGAAAAGGGCTTGGTGCTGATTT
ACTCCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGAGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAACTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACATTGTTCCTCAGACTTCCT
CAGTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT211635 WP_004026357.1 NZ_CP080298:c85003-84560 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT211635 NZ_CP106659:c66859-66593 [Klebsiella michiganensis]
ATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGATGCAGCTGCGGAAATCCTTCA
CAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATTCTCGACCGCCGTGTATTTAACT
TTAACGATGAGCAATATGCGGAGTTCATCGATATGCTTGATGCACCGGTTGCAGATGATCCCGTTATCGGAAAATTGCTG
GCAAGGAAACCTCAGTGGGACATGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A663AYG0


Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References