Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5391188..5391813 Replicon chromosome
Accession NZ_CP080297
Organism Klebsiella pneumoniae strain KP-C76

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K0E64_RS26630 Protein ID WP_002882817.1
Coordinates 5391188..5391571 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K0E64_RS26635 Protein ID WP_004150355.1
Coordinates 5391571..5391813 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E64_RS26615 (K0E64_26615) 5388554..5389456 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K0E64_RS26620 (K0E64_26620) 5389453..5390088 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K0E64_RS26625 (K0E64_26625) 5390085..5391014 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K0E64_RS26630 (K0E64_26630) 5391188..5391571 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K0E64_RS26635 (K0E64_26635) 5391571..5391813 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K0E64_RS26640 (K0E64_26640) 5392018..5392935 + 918 WP_002882812.1 alpha/beta hydrolase -
K0E64_RS26645 (K0E64_26645) 5392949..5393890 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K0E64_RS26650 (K0E64_26650) 5393935..5394372 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K0E64_RS26655 (K0E64_26655) 5394369..5395229 - 861 WP_002882807.1 virulence factor BrkB family protein -
K0E64_RS26660 (K0E64_26660) 5395223..5395822 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T211632 WP_002882817.1 NZ_CP080297:c5391571-5391188 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T211632 NZ_CP106658:c4804311-4804054 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCTTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT211632 WP_004150355.1 NZ_CP080297:c5391813-5391571 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT211632 NZ_CP106658:c4804043-4803492 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCAGGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References