Detailed information of TA system TA021163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 872834..873522
• Replicon: chromosome
• Accession: NC_006624
• Organism: Thermococcus kodakarensis KOD1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q5JIF0
• Locus tag: TK_RS04915
• Protein ID: WP_011249949.1
• Coordinates: 873115..873522 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TK_RS04910
• Protein ID: WP_232500621.1
• Coordinates: 872834..873118 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TK_RS04880 (TK0991)	868484..870223	+	1740	WP_011249942.1	carbon starvation protein A	-
TK_RS04885 (TK0992)	870264..870548	+	285	WP_011249943.1	hypothetical protein	-
TK_RS04890 (TK0993)	870560..870841	+	282	WP_011249944.1	hypothetical protein	-
TK_RS04895 (TK0994)	870852..871847	+	996	WP_011249945.1	ArsA family ATPase	-
TK_RS04900 (TK0995)	871837..872121	+	285	WP_011249946.1	iron-sulfur cluster assembly protein	-
TK_RS04905 (TK0996)	872123..872776	+	654	WP_011249947.1	hypothetical protein	-
TK_RS04910 (TK0997)	872834..873118	+	285	WP_232500621.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TK_RS04915 (TK0998)	873115..873522	+	408	WP_011249949.1	type II toxin-antitoxin system VapC family toxin	Toxin
TK_RS04920 (TK0999)	873525..874307	+	783	WP_011249950.1	alpha/beta hydrolase	-
TK_RS11815 (TK1000)	874708..874941	+	234	WP_011249951.1	hypothetical protein	-
TK_RS04930 (TK1001)	875044..875841	+	798	WP_048053699.1	hypothetical protein	-
TK_RS04935 (TK1002)	875838..876857	-	1020	WP_011249953.1	adenylosuccinate synthetase	-
TK_RS04940 (TK1003)	876973..877236	+	264	WP_143598683.1	hypothetical protein	-
TK_RS04945 (TK1004)	877272..878198	+	927	WP_011249955.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-127)

Antitoxin

• SpoVT-AbrB domain (10-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15693.00 Da
• Isoelectric Point: 4.4082

>T21163 WP_011249949.1 NC_006624:873115-873522 [Thermococcus kodakarensis KOD1]
MRLLIDTNLFVYLNANIDDELAERLDRFYAELVRENEAYTNVLVLDELIHVSKRKYGVSYPETISFIEDVVLPAVRVLPI
TFEDYLTAKEIILKYHLRPSDALHAATIQNNGLQAIVSEDEDFDKLPIKRLWLEV

Nucleotide

• Length: 408 bp

>T21163 NC_006624:873115-873522 [Thermococcus kodakarensis KOD1]
ATGAGGCTGCTCATTGACACCAACCTTTTTGTCTATTTAAACGCCAACATAGACGATGAACTCGCCGAGAGGCTCGATCG
CTTCTATGCAGAGCTGGTTAGAGAGAATGAAGCTTACACGAACGTCCTTGTTTTGGACGAGCTCATTCACGTTTCTAAAC
GAAAATACGGGGTGAGCTATCCTGAGACGATATCGTTCATAGAAGACGTTGTACTGCCTGCCGTTAGGGTGCTTCCCATC
ACCTTTGAGGATTACCTCACGGCCAAAGAAATAATTCTCAAGTACCACCTTCGCCCTTCAGATGCCCTCCACGCCGCGAC
GATTCAGAACAACGGCCTTCAGGCTATTGTGAGTGAGGATGAGGATTTTGATAAACTTCCCATTAAGAGGCTCTGGCTGG
AGGTGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10645.46 Da
• Isoelectric Point: 8.4825

>AT21163 WP_232500621.1 NC_006624:872834-873118 [Thermococcus kodakarensis KOD1]
VIPVVSIRLKVGPKGQIVIPKVFREAYGIKEGGEVIVEPREDALVIRRVPDTEELLKKLREYHKKRKGAKPAKLGELKGV
SLEDEFDEVWGIEE

Nucleotide

• Length: 285 bp

>AT21163 NC_006624:872834-873118 [Thermococcus kodakarensis KOD1]
GTGATACCGGTGGTTAGTATCCGCCTGAAAGTCGGCCCAAAGGGCCAGATAGTAATTCCCAAAGTCTTCAGGGAGGCCTA
CGGAATTAAGGAGGGCGGGGAGGTTATAGTGGAACCAAGGGAAGATGCACTCGTCATCAGGAGAGTGCCGGACACGGAAG
AACTCCTTAAAAAGCTCAGGGAGTACCACAAAAAGAGGAAAGGCGCGAAACCAGCTAAACTTGGGGAACTCAAAGGAGTG
AGCCTTGAAGACGAGTTCGACGAGGTCTGGGGGATTGAGGAATGA

Structures

Toxin

• AlphaFold DB: Q5JIF0