Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4194547..4195166 Replicon chromosome
Accession NZ_CP080297
Organism Klebsiella pneumoniae strain KP-C76

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K0E64_RS20890 Protein ID WP_002892050.1
Coordinates 4194948..4195166 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K0E64_RS20885 Protein ID WP_002892066.1
Coordinates 4194547..4194921 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E64_RS20875 (K0E64_20875) 4189699..4190892 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K0E64_RS20880 (K0E64_20880) 4190915..4194061 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
K0E64_RS20885 (K0E64_20885) 4194547..4194921 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K0E64_RS20890 (K0E64_20890) 4194948..4195166 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K0E64_RS20895 (K0E64_20895) 4195325..4195891 + 567 WP_002892030.1 maltose O-acetyltransferase -
K0E64_RS20900 (K0E64_20900) 4195863..4196003 - 141 WP_004147370.1 hypothetical protein -
K0E64_RS20905 (K0E64_20905) 4196024..4196494 + 471 WP_002892026.1 YlaC family protein -
K0E64_RS20910 (K0E64_20910) 4196469..4197920 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K0E64_RS20915 (K0E64_20915) 4198021..4198719 + 699 WP_002892021.1 GNAT family protein -
K0E64_RS20920 (K0E64_20920) 4198716..4198856 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K0E64_RS20925 (K0E64_20925) 4198856..4199119 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211628 WP_002892050.1 NZ_CP080297:4194948-4195166 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T211628 NZ_CP106658:955363-955773 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT211628 WP_002892066.1 NZ_CP080297:4194547-4194921 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT211628 NZ_CP106658:955116-955382 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References