21162

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 881902..882457 Replicon chromosome
Accession NC_006582
Organism Bacillus clausii KSM-K16

Toxin (Protein)


Gene name pemK Uniprot ID Q5WJV7
Locus tag ABC_RS04245 Protein ID WP_011245664.1
Coordinates 882107..882457 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A268RU80
Locus tag ABC_RS04240 Protein ID WP_050748939.1
Coordinates 881902..882102 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABC_RS04215 877286..877870 - 585 WP_142237599.1 YitT family protein -
ABC_RS04220 878001..878762 - 762 WP_011245659.1 rhomboid family intramembrane serine protease -
ABC_RS04225 878886..879239 + 354 WP_011245660.1 holo-ACP synthase -
ABC_RS04230 879359..880381 + 1023 WP_011245661.1 outer membrane lipoprotein carrier protein LolA -
ABC_RS04235 880527..881759 + 1233 WP_011245662.1 alanine racemase -
ABC_RS04240 881902..882102 + 201 WP_050748939.1 hypothetical protein Antitoxin
ABC_RS04245 882107..882457 + 351 WP_011245664.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ABC_RS04250 882727..883545 + 819 WP_011245665.1 RsbT co-antagonist protein RsbRA -
ABC_RS04255 883563..883919 + 357 WP_011245666.1 STAS domain-containing protein -
ABC_RS04260 883922..884353 + 432 WP_011245667.1 anti-sigma regulatory factor -
ABC_RS04265 884354..885370 + 1017 WP_011245668.1 PP2C family protein-serine/threonine phosphatase -
ABC_RS04270 885435..885764 + 330 WP_011245669.1 STAS domain-containing protein -
ABC_RS04275 885764..886246 + 483 WP_011245670.1 anti-sigma B factor RsbW -
ABC_RS04280 886206..886991 + 786 WP_011245671.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12845.93 Da        Isoelectric Point: 9.6233

>T21162 WP_011245664.1 NC_006582:882107-882457 [Bacillus clausii KSM-K16]
VIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNNIGNRFSPTVIVAAITAQIQKAKLPTHVEINASKYGFDRDSVILLEQV
RTIDKQRLTDKITHLDDKMMRSVDKALTISLGLIDF

Download         Length: 351 bp

>T21162 NC_006582:882107-882457 [Bacillus clausii KSM-K16]
GTGATAGTCAAACGCGGCGATGTGTATTTTGCAGATCTCTCCCCCGTTGTCGGCTCAGAACAAGGTGGGGTCAGGCCTGT
ACTCATCATACAAAATAATATCGGTAACCGATTTAGCCCGACAGTGATCGTAGCAGCTATTACAGCCCAAATCCAAAAAG
CAAAACTCCCAACACATGTGGAAATCAATGCGAGCAAATATGGGTTCGACCGGGATTCGGTCATTCTTCTTGAGCAAGTA
CGCACGATTGACAAACAACGACTGACAGATAAAATTACCCATCTTGACGACAAAATGATGAGGTCTGTTGACAAGGCACT
CACAATCAGTTTGGGATTAATTGATTTTTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7509.42 Da        Isoelectric Point: 4.4423

>AT21162 WP_050748939.1 NC_006582:881902-882102 [Bacillus clausii KSM-K16]
VDAVLEQEKWDNEENSNKRAKLKAEIRESMQQGYMEMAKINLTIASEAFLAEEEANHTLTRLVSGV

Download         Length: 201 bp

>AT21162 NC_006582:881902-882102 [Bacillus clausii KSM-K16]
GTGGATGCTGTGCTTGAGCAGGAAAAGTGGGACAACGAAGAAAACAGCAATAAGCGTGCAAAACTAAAAGCTGAAATTCG
TGAGTCAATGCAACAAGGCTATATGGAGATGGCGAAAATCAATCTTACAATTGCATCAGAAGCCTTTCTTGCTGAGGAGG
AAGCGAATCATACGCTGACGCGTCTCGTTAGTGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WJV7

Antitoxin

Source ID Structure
AlphaFold DB A0A268RU80

References