Detailed information of TA system TA211610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 207858..208497
• Replicon: chromosome
• Accession: NZ_CP080291
• Organism: Paeniclostridium sordellii strain FL-manatee

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KZ987_RS01295
• Protein ID: WP_055338275.1
• Coordinates: 208132..208497 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A446IEN4
• Locus tag: KZ987_RS01290
• Protein ID: WP_021126762.1
• Coordinates: 207858..208142 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ987_RS01260 (KZ987_01260)	203325..204719	+	1395	WP_055338271.1	F0F1 ATP synthase subunit beta	-
KZ987_RS01265 (KZ987_01265)	204722..204982	+	261	WP_021130261.1	ATP synthase F1 subunit epsilon	-
KZ987_RS01270 (KZ987_01270)	205184..205564	+	381	WP_055338272.1	holo-ACP synthase	-
KZ987_RS01275 (KZ987_01275)	205570..206031	+	462	WP_219840129.1	CBS domain-containing protein	-
KZ987_RS01280 (KZ987_01280)	206057..206647	+	591	WP_055343239.1	germination lipoprotein GerS	-
KZ987_RS01285 (KZ987_01285)	206664..207827	+	1164	WP_055343240.1	alanine racemase	-
KZ987_RS01290 (KZ987_01290)	207858..208142	+	285	WP_021126762.1	hypothetical protein	Antitoxin
KZ987_RS01295 (KZ987_01295)	208132..208497	+	366	WP_055338275.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KZ987_RS01300 (KZ987_01300)	208663..209490	+	828	WP_021130256.1	transketolase	-
KZ987_RS01305 (KZ987_01305)	209483..210415	+	933	WP_054629956.1	transketolase family protein	-
KZ987_RS01310 (KZ987_01310)	210542..210958	+	417	WP_021126758.1	hypothetical protein	-
KZ987_RS01315 (KZ987_01315)	211087..213468	+	2382	WP_057539243.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13495.64 Da
• Isoelectric Point: 7.9717

>T211610 WP_055338275.1 NZ_CP080291:208132-208497 [Paeniclostridium sordellii]
VSSNSLDIKRGYLYYADLSPVVGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHIEISSNEYGLNKDSVI
LLEQIRTIDKKRLREKIGYLDECMMEKVNNSLLISLGLFQL

Nucleotide

• Length: 366 bp

>T211610 NZ_CP106654:730765-731250 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10700.15 Da
• Isoelectric Point: 4.4179

>AT211610 WP_021126762.1 NZ_CP080291:207858-208142 [Paeniclostridium sordellii]
VRNIGKEKIVFNLPDSLISEVDHIVQIENTNREDFAKAAFQFYITQKKKFDLKESMIKGYKEMGQINLTLAELGMTNEVS
SDDDLEGIIAQGEF

Nucleotide

• Length: 285 bp

>AT211610 NZ_CP106654:730475-730768 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A446IEN4