Detailed information of TA system TA211604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1017397..1017983
• Replicon: chromosome
• Accession: NZ_CP080289
• Organism: Pseudomonas aeruginosa strain PA2207

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K0E62_RS04895
• Protein ID: WP_003120987.1
• Coordinates: 1017397..1017696 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K0E62_RS04900
• Protein ID: WP_003448662.1
• Coordinates: 1017693..1017983 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E62_RS04875 (K0E62_04875)	1012510..1013043	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
K0E62_RS04880 (K0E62_04880)	1013033..1014358	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
K0E62_RS04885 (K0E62_04885)	1014362..1016071	-	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
K0E62_RS04890 (K0E62_04890)	1016071..1017195	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
K0E62_RS04895 (K0E62_04895)	1017397..1017696	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E62_RS04900 (K0E62_04900)	1017693..1017983	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K0E62_RS04905 (K0E62_04905)	1018054..1018398	-	345	WP_016851612.1	hypothetical protein	-
K0E62_RS04910 (K0E62_04910)	1018539..1020515	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
K0E62_RS04915 (K0E62_04915)	1020512..1022401	-	1890	WP_196994395.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	958800..1046012	87212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T211604 WP_003120987.1 NZ_CP080289:1017397-1017696 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T211604 NZ_CP104992:3288721-3289053 [Rhizobium sp. CC-CFT758]
ATGATGCGCTTCCTGGTGGACGCACAATTGCCACCTGCTCTTGCCCGCTGGCTGGTCGATCAGGGCCATGAAGCCGGCCA
TGTCGTCGATTTCAATCTTCAATCTGCGGCTGATCGCGAGATATGGGACTTCGCGGTCCAAAGGGACGCGGTTATCATCA
CCAAGGATGAGGATTTCGCCCAGCGAAGAGCCTTGACGAAAGACGGCCCAACGATCGTTTGGGTGCGCCTACCGAATTCC
CGCCGAAGCGACCTCTTGCAGTGGTTCGAGAAAGCTCTCGACGATATCGTCGCCGCGTTGGAACGCGGCGAGACGATCGT
CGAGGTCATCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT211604 WP_003448662.1 NZ_CP080289:1017693-1017983 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT211604 NZ_CP104992:3288500-3288724 [Rhizobium sp. CC-CFT758]
ATGTCCGAGCTTCATCGCATCACATTCGATCCGCATGTCTCTTCCGGTCGTCCGACGATCCGCGGCATGCGTATCCGCGT
GAAGGACATTCTGGAGCTTTTGGCCGCTGGTGCAAGCCATGAGGAAATCCTGGAGGATTATCCGACACTCGAACCGGCTG
ATATCCGCGCGGCGCTTGAATATGCAGCACGCCAGAGCGACCATACCGTTCTGCGCGTGGCATGA

Structures

Toxin

• AlphaFold DB: G8CP73