Detailed information of TA system TA021160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4256468..4257109
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB_RS20015
• Protein ID: WP_011239860.1
• Coordinates: 4256699..4257109 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: Q5NXJ8
• Locus tag: EB_RS20010
• Protein ID: WP_011239859.1
• Coordinates: 4256468..4256698 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS19990	4251638..4252582	+	945	WP_011239855.1	flocculation-associated PEP-CTERM protein PepA	-
EB_RS19995	4252665..4254191	+	1527	WP_041646646.1	hypothetical protein	-
EB_RS20000	4254253..4255326	-	1074	WP_011239857.1	nitroreductase family protein	-
EB_RS20005	4255319..4256206	-	888	WP_041646647.1	ThiF family adenylyltransferase	-
EB_RS20010	4256468..4256698	+	231	WP_011239859.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EB_RS20015	4256699..4257109	+	411	WP_011239860.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB_RS20020	4257305..4259197	-	1893	WP_011239861.1	propionate--CoA ligase	-
EB_RS20025	4259522..4260520	-	999	WP_011239863.1	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15396.53 Da
• Isoelectric Point: 8.1014

>T21160 WP_011239860.1 NC_006513:4256699-4257109 [Aromatoleum aromaticum EbN1]
MSVRYLLDTNICIYIAKHNPPEVRQRFARHAASELAMSVITLGELRFGAEKSQARDKALAAIQRLEATVRIAPLTESVGE
HYGQIRANLQHKGQPIGNNDLWLAAHARAEGWILVTNNEREFVRVEGLQVENWVDR

Nucleotide

• Length: 411 bp

>T21160 NC_006513:4256699-4257109 [Aromatoleum aromaticum EbN1]
ATGTCGGTCCGCTATTTGCTCGACACGAACATCTGCATCTACATCGCGAAGCACAACCCGCCGGAAGTTCGCCAGCGCTT
CGCCCGCCACGCTGCATCCGAACTCGCGATGTCCGTCATCACGCTCGGCGAACTGCGCTTCGGCGCGGAGAAGAGCCAGG
CCCGCGACAAGGCGCTCGCTGCGATTCAAAGGCTGGAAGCGACGGTCCGGATCGCGCCACTGACGGAGTCGGTCGGCGAG
CACTACGGCCAGATCCGCGCGAATCTCCAGCACAAGGGCCAACCGATCGGCAACAACGACCTGTGGCTGGCCGCCCACGC
GCGCGCCGAAGGTTGGATCCTCGTGACAAACAACGAACGCGAATTCGTCCGCGTAGAAGGACTGCAAGTCGAAAACTGGG
TAGACCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8676.88 Da
• Isoelectric Point: 4.7863

>AT21160 WP_011239859.1 NC_006513:4256468-4256698 [Aromatoleum aromaticum EbN1]
MVYARVFRSGNSQAVRLPKEFRLDTDRVEIFRRGDEIVLRELPGNAAAIFDALIELPVNFMSEGRNDVAPQEREAL

Nucleotide

• Length: 231 bp

>AT21160 NC_006513:4256468-4256698 [Aromatoleum aromaticum EbN1]
ATGGTTTACGCCCGCGTCTTCCGCTCCGGCAACAGCCAGGCGGTCCGCCTGCCGAAGGAGTTTCGGCTCGATACGGACCG
GGTCGAAATCTTCCGCCGCGGCGATGAAATCGTGCTGCGCGAACTGCCCGGCAATGCGGCCGCGATTTTCGATGCGCTAA
TCGAGCTGCCCGTCAACTTCATGTCTGAAGGGCGCAACGATGTCGCGCCACAGGAGCGGGAGGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: Q5NXJ8