Detailed information of TA system TA021158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3599327..3600435
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q5NZC0
• Locus tag: EB_RS16990
• Protein ID: WP_011239254.1
• Coordinates: 3599683..3600435 (+)
• Length: 251 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EB_RS16985
• Protein ID: WP_041646544.1
• Coordinates: 3599327..3599686 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS16955	3594505..3595083	-	579	WP_011239246.1	ferritin-like domain-containing protein	-
EB_RS16960	3595278..3595973	-	696	WP_011239248.1	RNA polymerase sigma factor	-
EB_RS16965	3596028..3596642	-	615	WP_011239249.1	hypothetical protein	-
EB_RS16970	3596720..3597052	-	333	WP_011239250.1	cupin domain-containing protein	-
EB_RS16975	3597061..3597813	-	753	WP_011239251.1	SDR family oxidoreductase	-
EB_RS16980	3598085..3599242	+	1158	WP_011239252.1	PQQ-dependent sugar dehydrogenase	-
EB_RS16985	3599327..3599686	+	360	WP_041646544.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
EB_RS16990	3599683..3600435	+	753	WP_011239254.1	RES family NAD+ phosphorylase	Toxin
EB_RS23450	3600547..3600813	-	267	WP_011239255.1	Txe/YoeB family addiction module toxin	-
EB_RS23455	3600807..3601049	-	243	WP_011239256.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EB_RS17000	3601271..3601525	-	255	WP_041646546.1	hypothetical protein	-
EB_RS17005	3601595..3602719	-	1125	WP_011235911.1	ISAs1-like element ISAzo3 family transposase	-
EB_RS24385	3603159..3603425	-	267	WP_157866646.1	hypothetical protein	-
EB_RS17010	3603463..3603765	-	303	WP_041646547.1	hypothetical protein	-
EB_RS24390	3603858..3604433	-	576	WP_157866647.1	hypothetical protein	-
EB_RS23460	3604653..3604886	+	234	WP_083782991.1	phage integrase N-terminal SAM-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3601595..3602719	1124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (60-210)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (8-64)

Sequences

Toxin

Protein

• Length: 251 a.a.
• Molecular weight: 27813.73 Da
• Isoelectric Point: 5.8790

>T21158 WP_011239254.1 NC_006513:3599683-3600435 [Aromatoleum aromaticum EbN1]
VTDIWAAVGAEAPVVELAGNLVRLVESQEQIATNRLVDTLEEQALLESLLESSKPPLPHAARGLHYLLATPFRYPPLRHG
SRFGGHFEPSLFYAAFDTATVLAESAYYRFVFWHGMTVAPASPLASQHTLFGAPWRCARGVQLQCPPFDRWREQLTDPAN
YAATQRLGTAMRQAGVEAFEYVSARDPAGGLNVALFTPMALAARRPDFMQAWLAETDAGEVRFYCQEERLVHRFPLWQFL
VAGELPMPAV

Nucleotide

• Length: 753 bp

>T21158 NC_006513:3599683-3600435 [Aromatoleum aromaticum EbN1]
GTGACGGACATCTGGGCGGCGGTCGGCGCAGAAGCGCCGGTCGTCGAGCTCGCAGGCAATCTCGTGCGGCTCGTCGAGAG
CCAGGAGCAGATCGCGACGAACCGCCTCGTCGATACCCTCGAGGAACAGGCGCTCCTCGAATCCCTGCTGGAAAGCTCGA
AGCCGCCGCTGCCGCACGCGGCCCGCGGCCTGCATTACCTGCTGGCCACCCCGTTCCGTTATCCGCCGCTGCGCCACGGC
TCGCGTTTCGGCGGGCACTTCGAGCCCAGCCTGTTCTACGCGGCGTTCGATACGGCAACGGTGCTGGCCGAGTCGGCCTA
TTACCGCTTCGTCTTCTGGCACGGCATGACCGTCGCGCCGGCGTCCCCCCTGGCGTCCCAGCACACGCTGTTCGGCGCGC
CGTGGCGCTGCGCCCGCGGCGTGCAACTGCAATGCCCGCCGTTCGATCGGTGGCGTGAACAGCTCACCGACCCCGCGAAT
TACGCCGCGACCCAGCGACTCGGCACGGCGATGCGGCAAGCCGGCGTCGAAGCTTTCGAATACGTCTCCGCGCGCGACCC
GGCCGGCGGCCTCAACGTCGCGCTATTCACTCCGATGGCGCTTGCCGCCCGGCGGCCGGACTTCATGCAGGCGTGGCTCG
CGGAAACGGACGCCGGCGAAGTGCGCTTCTACTGCCAGGAAGAGCGGCTCGTGCATCGCTTCCCGCTCTGGCAGTTCCTC
GTCGCGGGCGAGCTGCCGATGCCGGCGGTATAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 12749.85 Da
• Isoelectric Point: 10.3723

>AT21158 WP_041646544.1 NC_006513:3599327-3599686 [Aromatoleum aromaticum EbN1]
MSVAALRKPDATQVVTKALLNAGKEMGLTQAALGVVIGKDRTAISRNGIDPDSKAGELALLLIRAYRALFALVGGDAAQM
KHWMHTDNHHVGGVPAEQIRTVQGLLRVVEYLDAIRGRL

Nucleotide

• Length: 360 bp

>AT21158 NC_006513:3599327-3599686 [Aromatoleum aromaticum EbN1]
ATGAGCGTTGCCGCCCTTCGCAAACCCGATGCGACACAGGTCGTCACCAAGGCCCTGCTCAACGCCGGCAAGGAAATGGG
GCTGACGCAGGCGGCGCTGGGCGTGGTCATCGGCAAGGACCGCACCGCGATCAGCCGCAACGGCATCGACCCCGACAGCA
AGGCGGGAGAGCTCGCGCTGCTGCTGATCCGCGCGTATCGCGCGCTGTTCGCGCTCGTCGGGGGCGACGCCGCGCAGATG
AAGCACTGGATGCACACCGACAACCACCACGTCGGCGGCGTCCCGGCCGAGCAGATTCGCACCGTCCAGGGTTTGCTGCG
CGTCGTCGAATACCTCGACGCGATCCGCGGCCGGCTGTGA

Structures

Toxin

• AlphaFold DB: Q5NZC0