Detailed information of TA system TA211575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 4922369..4924064
• Replicon: chromosome
• Accession: NZ_CP080280
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF20

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KZ794_RS22725
• Protein ID: WP_003112901.1
• Coordinates: 4923195..4924064 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KZ794_RS22720
• Protein ID: WP_003112902.1
• Coordinates: 4922369..4923202 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ794_RS22700 (KZ794_22705)	4917600..4919084	-	1485	WP_003453562.1	aldehyde dehydrogenase	-
KZ794_RS22705 (KZ794_22710)	4919096..4919899	-	804	WP_003130116.1	aspartate dehydrogenase	-
KZ794_RS22710 (KZ794_22715)	4919910..4921592	-	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
KZ794_RS22715 (KZ794_22720)	4921585..4922382	-	798	WP_003112903.1	SDR family oxidoreductase	-
KZ794_RS22720 (KZ794_22725)	4922369..4923202	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KZ794_RS22725 (KZ794_22730)	4923195..4924064	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KZ794_RS22730 (KZ794_22735)	4924073..4924603	-	531	WP_003112900.1	cupin domain-containing protein	-
KZ794_RS22735 (KZ794_22740)	4924617..4925378	-	762	WP_023083383.1	SDR family oxidoreductase	-
KZ794_RS22740 (KZ794_22745)	4925384..4926160	-	777	WP_003112898.1	ABC transporter permease	-
KZ794_RS22745 (KZ794_22750)	4926157..4927164	-	1008	WP_023083382.1	ABC transporter substrate-binding protein	-
KZ794_RS22750 (KZ794_22755)	4927205..4928056	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T211575 WP_003112901.1 NZ_CP080280:c4924064-4923195 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T211575 NZ_CP104972:c53488-53267 [Phyllobacterium zundukense]
ATGTTCGGGGTGATTCAAGCTCGGCGATACAAAGACGAACTGGACTACTGCTTCAAATTATTGGCTGACACGCCGCATAT
GGGACGATTGGCAGAAACGTTGGGATCTGGTGTAAGGCGGCACGAGCACAAGAGTCATGTTATCCTGTACGAACAGGAAG
CAAATGGCATCTTGGTTTTGGCACTTGTACCCGGTCGGAGCGTTCGCCAACTGAAATTATAG

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT211575 WP_003112902.1 NZ_CP080280:c4923202-4922369 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT211575 NZ_CP104972:c53816-53550 [Phyllobacterium zundukense]
ATGGCGACCATGACGGTATCATTGCCCGATCAAATGAAAGACTGGATAGAGACGCAAATCCGGAAGGGAGAATATGCCAG
CACCAGTGACTACGTGCGCGATTTGGTGCGCCGGGATCGTTCCCGGCGAGGAAAGGAATTCACGCTGGATGAACTGCGTC
AACTGGTGGCCGATGCAAAGGCTAGCGGCGTTGGAACACGGAGCATGGACGATATTTTTGCTCAGGCGGAACAGATTGCC
AGCGCCCGTGGCTTAGGACGTGAGTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1