Detailed information of TA system TA211573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3795862..3796904
• Replicon: chromosome
• Accession: NZ_CP080280
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF20

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: KZ794_RS17290
• Protein ID: WP_003153636.1
• Coordinates: 3795862..3796437 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: KZ794_RS17295
• Protein ID: WP_003050245.1
• Coordinates: 3796434..3796904 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ794_RS17265 (KZ794_17260)	3792300..3793025	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
KZ794_RS17270 (KZ794_17265)	3793064..3793966	-	903	WP_003090158.1	CBASS oligonucleotide cyclase	-
KZ794_RS17275 (KZ794_17270)	3793966..3794466	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
KZ794_RS17280 (KZ794_17275)	3794463..3794933	-	471	WP_003090160.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
KZ794_RS17285 (KZ794_17280)	3794930..3795844	-	915	WP_016852809.1	AAA family ATPase	-
KZ794_RS17290 (KZ794_17285)	3795862..3796437	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
KZ794_RS17295 (KZ794_17290)	3796434..3796904	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
KZ794_RS17300 (KZ794_17295)	3797108..3797491	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
KZ794_RS17305 (KZ794_17300)	3797488..3797721	+	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
KZ794_RS17310 (KZ794_17305)	3797738..3798097	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
KZ794_RS17315 (KZ794_17310)	3798109..3798507	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
KZ794_RS17320 (KZ794_17315)	3798504..3799196	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
KZ794_RS17325 (KZ794_17320)	3799193..3800104	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
KZ794_RS17330 (KZ794_17325)	3800094..3801512	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3749746..3871479	121733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T211573 WP_003153636.1 NZ_CP080280:c3796437-3795862 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T211573 NZ_CP104970:122227-123132 [Phyllobacterium zundukense]
ATGGCGCGTGAACCGAAGAAAAACGTCGGGGCATCCGTGCGCGCCCGGCTGCTTGCGCGGTCACGCACAGAGCGCGTGGA
CTTTCAGATCCTGCTGACGCGCTACGCGCTCGAAAGGCTCCTGTACCGGCTGAGCCTATCCGCGCATCGCGACCGCTTCA
TCCTAAAGGGCGCGATGCTCTTCGTAACGTGGGTCGCCGACCCGTTTCGGCCGACGCGTGACCTCGACCTGCTGGGTTAC
GGTGAGAACAGCCCGAAGGCCATCGCCGAAACTTTCCATGTCATTTGCACGCAGCCGGTCGACGATGACGGCGTCGTTTT
CGACGTCGATGGGCTCGAAGCCGTGCCGATCCGTGAGGAAGTCGAATACGGCGGCGTTCGTGTCCGGACCACTGCCGCTA
TCGCAGGCGCGCGCATTCCCATTCAGGTGGACATAGGCTTCGGTGATGCCGTCACGCCTGGACCGGTCGACATAGAATAT
CCGTCGCTGCTCGATGCGCCGGCGCCGCGCCTGCGGGCCTATCCGGTCGCGACCGTGGTCGCCGAGAAATTCGAGGCGCT
GGTAACGCTCGGCATGGCAAATAGCCGCCTGAAGGATTTTTACGATCTCTGGTGGATTGCCGAGACGTTCGAACTTGAGC
GGCCCACCCTTGTAGAGGCCGTGCGCCAGACATTTGCTAGAAGAGGGACGGAACTTCCCAAGGAGAGACCGACGGGTCTC
AGCGATGCCTACGCGGAAGTATGGGATCGTCAGTGGCGGGCCTTTCTTGGTCGCGAACGCATGGCGGCCGCGCCGCCAAG
CTTGGCCACGGTAGTGGGGGATCTCGGGCACCTTCTGCTGCCCTTGACAGAGCCCCTCGATGGCAATTGGCGTTGGAAGC
CGCGCGAGGGATGGATACAGCCGTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT211573 WP_003050245.1 NZ_CP080280:c3796904-3796434 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT211573 NZ_CP104970:121623-122237 [Phyllobacterium zundukense]
ATGGCCGACACCCAACGGGCTCGCGCGCTCGAACTCCTGAAAGCTCGGCACATGCTGCGGCTGAAGGATTTCGCCGCGCA
CGGAATTGGCTCGGAGACGCTCGCGCGTCTCATACGAGATGAAGAAGTCGTTCGTCCGGCGCGCGGTCTCTACCAGCTTC
CCGACGCTGCGACGGACGTAAGTCATGCCCTGGCCGAGGCATCAGCGCTGGTGCCGAAAGGGATCGTCTGCCTGACCTCG
GCGCTGCAATTTCATGAGCTGACGCTGCAAATGCCGTCGGCGGTCTGGATGGCCATTGATCGCACGGCTTGGCGCCCCAA
GATCGACTATCCGCCGATCCGGTTCGTACGCTTCACCGGGTTCGCTCTCACCGAGGGCGTCGAACGGCATCGCATCGAGG
GCGTCGAGGTGCCGATCACAAATCCGGCGCGCTCGATCGTCGATTGCTTCCGCTATCGATCGAAAGTCGGCCTCGACGTT
GCGATGGAGGGGCTGCGCGAGGGCCTTCGGCGGCGCAAGACAACAAGCGACGAACTCTGGACATATGCGAAGAAGGCCAG
GATCTGGTCGACCATGCGACCCTACGTCGAAGCGACGGTGGCCGATGGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: I3TV68