Detailed information of TA system TA211570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1066779..1067365
• Replicon: chromosome
• Accession: NZ_CP080280
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF20

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KZ794_RS04870
• Protein ID: WP_003120987.1
• Coordinates: 1066779..1067078 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KZ794_RS04875
• Protein ID: WP_003448662.1
• Coordinates: 1067075..1067365 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ794_RS04850 (KZ794_04850)	1061892..1062425	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
KZ794_RS04855 (KZ794_04855)	1062415..1063740	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KZ794_RS04860 (KZ794_04860)	1063744..1065453	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
KZ794_RS04865 (KZ794_04865)	1065453..1066577	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
KZ794_RS04870 (KZ794_04870)	1066779..1067078	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ794_RS04875 (KZ794_04875)	1067075..1067365	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KZ794_RS04880 (KZ794_04880)	1067436..1067780	-	345	WP_016851612.1	hypothetical protein	-
KZ794_RS04885 (KZ794_04885)	1067921..1069897	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KZ794_RS04890 (KZ794_04890)	1069894..1071783	-	1890	WP_016851610.1	hypothetical protein	-
KZ794_RS04895 (KZ794_04895)	1072005..1072214	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	999867..1094344	94477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T211570 WP_003120987.1 NZ_CP080280:1066779-1067078 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T211570 NZ_CP104967:651218-651643 [Phyllobacterium sp. A18/5-2]
ATGATCCTCATCGACACCAACGTGATTTCGGAGCCGTGGAGGCTCACGCCCGATGCGCGTGTGCTGGCATGGATCGACGC
CCAGGCCATCGAAACCCTATACCTGTCGGCGGTCACTATCGCCGAGGTGGGTTTCGGTATTGCCTCGATGCCCGCCGGCA
AGCGCCGCACGACCTTGCATGATCGCTTGGAAAACGAGGTGCTGCCGCTGTTTGAAGGCCGCCTGCTGCCCTTCGATTTA
GACGCTTCGCAAGCCTATGCCGACCTGATGGCGCAGGCGAAGGCGGCAGGAAGAGCGATCGGCAAGGCAGACGGCTACAT
CGCCGCTACGGCCGCCGCGCACGGCCTCATGGTGGCTACTCGCGACACCAGCCCGTTTGAAGCCGCTGGCGTTCCCATCA
TCAATCCTTGGGAGACCAGCGACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT211570 WP_003448662.1 NZ_CP080280:1067075-1067365 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT211570 NZ_CP104967:650967-651221 [Phyllobacterium sp. A18/5-2]
ATGGCGACACTTACCGTAAGAAATCTGCCCGAAGAGGTGCACCGCGCGCTCCGGATGCGAGCGGCCACGCACGGCCGCAG
CACTGAGGCCGAAGTTAGAGACATTCTGGAAACGACTGTCCGGCCGCCCGAGCGCGTCAAGCTCGGCTCGTTGCTGGCGT
CCATCGCCCGCGACGCGGGTTTCAACGACGCCGAAGCGGAGCAGTTCGACCAGCTCCGCGATAAGACCCCGGCCGAGCCG
GTGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: G8CP73