211567

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4457641..4458182 Replicon chromosome
Accession NZ_CP080274
Organism Mycolicibacterium smegmatis strain Jucho

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag KZ780_RS21210 Protein ID WP_003895797.1
Coordinates 4457853..4458182 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag KZ780_RS21205 Protein ID WP_011729843.1
Coordinates 4457641..4457850 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ780_RS21180 (KZ780_21040) 4452912..4453682 - 771 WP_011729837.1 SDR family oxidoreductase -
KZ780_RS21185 4454219..4454620 - 402 Protein_4207 cupin domain-containing protein -
KZ780_RS21190 (KZ780_21050) 4454650..4455570 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
KZ780_RS21195 (KZ780_21055) 4455567..4455851 - 285 WP_011729841.1 hypothetical protein -
KZ780_RS21200 (KZ780_21060) 4456113..4457480 + 1368 WP_014878037.1 dihydrolipoyl dehydrogenase -
KZ780_RS21205 (KZ780_21065) 4457641..4457850 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
KZ780_RS21210 (KZ780_21070) 4457853..4458182 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZ780_RS21215 (KZ780_21075) 4458225..4458788 - 564 WP_014878038.1 helix-turn-helix domain-containing protein -
KZ780_RS21220 (KZ780_21080) 4458894..4459829 + 936 WP_003895799.1 alpha/beta fold hydrolase -
KZ780_RS21225 (KZ780_21085) 4459826..4461538 + 1713 WP_239779513.1 NAD(P)/FAD-dependent oxidoreductase -
KZ780_RS21235 (KZ780_21095) 4461755..4462135 + 381 WP_029104585.1 phage holin family protein -
KZ780_RS21240 (KZ780_21100) 4462153..4463178 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T211567 WP_003895797.1 NZ_CP080274:4457853-4458182 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T211567 NZ_CP104966:2794397-2794651 [Phyllobacterium sp. A18/5-2]
ATGATCGAGGAATATTTGCTGGCGGTCTGCGAAGACGGGACATCTTCAGAGATCGCGCGTGCGTTGGGGGCAATAGCTCG
TGCAAGAGGCATTACCGAGCTTGCCCACAAGACCGGTCTCACACGTCAGGCGCTCTACAAAGCCTTGAGCGGTGAGGGCA
ATCCGGAACTTGCGACGATTACTAAAGTCGCCGAAGCGCTTGGCCTCCGATTGACTTTGGTGCGTGCACCGAAAAACAAG
CAAGACGCCGCGTAG

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT211567 WP_011729843.1 NZ_CP080274:4457641-4457850 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT211567 NZ_CP104966:2794041-2794349 [Phyllobacterium sp. A18/5-2]
TTGAATTATTCAATTGAAAAGACAGATACATTTTTAAACTGGATTGAGGGTTTGCAGGATCTGCGAGCGCGGGCAAGAAT
AATGTCGCGGCTGGACCGGCTGGCTGATGGAAATTTCGGCGATTACAAACTTCTTGGTGAGGATGTCGGAGAGATGCGCA
TTGATTGCGGACCAGGTTATCGGCTCTACTTCACCAAGCAAGGTGGCATTTTGATTATTCTGTTATGCGGTGGCGACAAG
AGCACGCAGAGCAGGGACATTAGAAAAGCGAAGGCAATGGCGCGTGATTTGAAGGAGATACGATCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References