Detailed information of TA system TA211563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5751460..5752101
• Replicon: chromosome
• Accession: NZ_CP080273
• Organism: Mycolicibacterium smegmatis strain Nishi

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KZ781_RS27335
• Protein ID: WP_011730672.1
• Coordinates: 5751460..5751903 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A2U9PXD6
• Locus tag: KZ781_RS27340
• Protein ID: WP_003897042.1
• Coordinates: 5751913..5752101 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ781_RS27315 (KZ781_27150)	5746944..5747681	+	738	WP_011730668.1	GntR family transcriptional regulator	-
KZ781_RS27320 (KZ781_27155)	5747683..5749212	+	1530	WP_233046689.1	cyclohexanecarboxylate-CoA ligase	-
KZ781_RS27325 (KZ781_27160)	5749213..5749986	+	774	WP_239707327.1	SDR family oxidoreductase	-
KZ781_RS27330 (KZ781_27165)	5749983..5751455	+	1473	WP_239707328.1	long-chain fatty acid--CoA ligase	-
KZ781_RS27335 (KZ781_27170)	5751460..5751903	-	444	WP_011730672.1	SRPBCC family protein	Toxin
KZ781_RS27340 (KZ781_27175)	5751913..5752101	-	189	WP_003897042.1	antitoxin	Antitoxin
KZ781_RS27345 (KZ781_27180)	5752127..5754514	-	2388	WP_239707330.1	cation-translocating P-type ATPase	-
KZ781_RS27350 (KZ781_27185)	5754528..5756135	-	1608	WP_239707332.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T211563 WP_011730672.1 NZ_CP080273:c5751903-5751460 [Mycolicibacterium smegmatis]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T211563 NZ_CP104965:c3515562-3515293 [Devosia neptuniae]
TTGGCCTGGACGATTGAGGTAGACGAACATGCCGCCCGGCAATTCGCGCGCCTCGATCGGCAGGATGCCACGCGAATTCG
CAACTATTTGCGGGACCGCGTCGCGACCCTCGATAATCCGCGGCAATTGGGCAAGGCATTGGCCGGCTCCAAGCTCGGAG
AGCTTTGGCGCTATCGCGTCGGAGATTTCCGGATCATCTGCGATCTACAGGACGGACGCCTTGTCATCCTAGTGCTGGGT
GTCGGTCATCGCAGCCAGGTTTATCGCTAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6886.64 Da
• Isoelectric Point: 4.6906

>AT211563 WP_003897042.1 NZ_CP080273:c5752101-5751913 [Mycolicibacterium smegmatis]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIRKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT211563 NZ_CP104965:c3515779-3515546 [Devosia neptuniae]
ATGTCCAAGCAAACGGCCGTTCGGCTTCCCGACGAACTCTATGATCGGCTGCAGTCCTTGGCGAATCGCACGGGCCGCAC
GGCGACGTTCTATATTCGCGAAGCCGTGGAGGAGCATGTCCAGGATCTCGAGGACCTGTACCTGGCCGAGGACGTGCTCA
AGCGCCGCGCGGCAGGCGACAACAAAACCACCAGCCTGGATGAACTGAGCAAGCGTCTTGGCCTGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PXD6